Identification
HMDB Protein ID CDBP05416
Secondary Accession Numbers Not Available
Name Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
Description Not Available
Synonyms
  1. 60S ribosomal protein L8 histidine hydroxylase
  2. Histone lysine demethylase NO66
  3. Myc-associated protein with JmjC domain
  4. Nucleolar protein 66
  5. Ribosomal oxygenase NO66
  6. hsNO66
  7. ROX
Gene Name NO66
Protein Type Enzyme
Biological Properties
General Function Not Available
Specific Function Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.
GO Classification
Biological Process
negative regulation of transcription, DNA-dependent
negative regulation of osteoblast differentiation
transcription, DNA-dependent
Cellular Component
nucleolus
nucleus
nucleoplasm
Molecular Function
iron ion binding
histone demethylase activity (H3-K4 specific)
histone demethylase activity (H3-K36 specific)
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
Cellular Location Not Available
Pathways
Gene Properties
Chromosome Location 14
Locus 14q24.3
SNPs Not Available
Gene Sequence Not Available
Protein Properties
Number of Residues Not Available
Molecular Weight 71084.97
Theoretical pI 6.455
Pfam Domain Function Not Available
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence
>gi|106879206|ref|NP_078920.2| bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 [Homo sapiens]
MDGLQASAGPLRRGRPKRRRKPQPHSGSVLALPLRSRKIRKQLRSVVSRMAALRTQTLPS
ENSEESRVES
GenBank ID Protein Not Available
UniProtKB/Swiss-Prot ID Q9H6W3
UniProtKB/Swiss-Prot Entry Name Not Available
PDB IDs
GenBank Gene ID Not Available
GeneCard ID Not Available
GenAtlas ID Not Available
HGNC ID HGNC:20968
References
General References Not Available