Cannabis Compound Database: Showing Protein Poly(U)-binding-splicing factor PUF60 (CDBP04926)

Identification Biological properties Gene properties Protein properties External links References XML Show 0 compounds

Identification

HMDB Protein ID

CDBP04926

Secondary Accession Numbers

Not Available

Name

Poly(U)-binding-splicing factor PUF60

Description

Not Available

Synonyms

60 kDa poly(U)-binding-splicing factor
FBP-interacting repressor
FUSE-binding protein-interacting repressor
Ro-binding protein 1
RoBP1
Siah-BP1
Siah-binding protein 1

Gene Name

PUF60

Protein Type

Enzyme

Biological Properties

General Function

Involved in nucleotide binding

Specific Function

DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA

GO Classification

Function
binding
nucleotide binding
nucleic acid binding

Cellular Location

Nucleus

Pathways

Not Available

Gene Properties

Chromosome Location

Chromosome:8

Locus

8q24.3

SNPs

PUF60

Gene Sequence

>1680 bp
ATGGCGACGGCGACCATAGCTCTCCAGGTCAATGGCCAGCAAGGAGGGGGGTCCGAGCCG
GCGGCGGCGGCGGCAGTGGTGGCAGCGGGAGACAAATGGAAACCTCCACAGGGCACAGAC
TCCATCAAGATGGAGAACGGGCAGAGCACAGCCGCCAAGCTGGGGCTGCCTCCCCTGACG
CCCGAGCAGCAGGAGGCCCTTCAGAAGGCCAAGAAGTACGCCATGGAGCAGAGCATCAAG
AGTGTGCTGGTGAAGCAGACCATCGCGCACCAGCAGCAGCAGCTCACCAACCTGCAGATG
GCAGCAGTGACAATGGGCTTTGGAGATCCTCTCTCACCTTTGCAATCGATGGCGGCTCAG
CGGCAGCGAGCGCTGGCCATCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTG
GGGGAGGACACCATCCGCCAGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATG
TCCTGGGACTCCGTCACCATGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCC
GAAGCTGCACAGCTGGCCTTGGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATC
AAGGTGGGCAGACCCAGCAACATAGGGCAGGCCCAGCCCATCATAGACCAGTTGGCTGAG
GAGGCACGGGCCTTCAACCGCATCTACGTGGCCTCTGTGCACCAGGACCTCTCAGACGAT
GACATCAAGAGCGTGTTTGAGGCCTTTGGCAAGATCAAGTCCTGCACACTGGCCCGGGAC
CCCACAACTGGCAAGCACAAGGGCTACGGCTTCATTGAGTACGAGAAGGCCCAGTCGTCC
CAAGATGCTGTGTCTTCCATGAACCTCTTTGACCTGGGTGGCCAGTACTTGCGGGTGGGC
AAGGCTGTCACACCGCCCATGCCCCTACTCACACCAGCCACGCCTGGAGGCCTCCCACCT
GCCGCTGCTGTGGCAGCTGCTGCAGCCACTGCCAAGATCACAGCTCAGGAAGCAGTGGCC
GGAGCAGCGGTGCTGGGTACCCTGGGCACACCTGGACTGGTGTCCCCAGCACTGACCCTG
GCCCAGCCCCTGGGCACTTTGCCCCAGGCTGTCATGGCTGCCCAGGCACCTGGAGTCATC
ACAGGTGTGACCCCAGCCCGTCCTCCTATCCCGGTCACCATCCCCTCGGTGGGAGTGGTG
AACCCCATCCTGGCCAGCCCTCCAACGCTGGGTCTCCTGGAGCCCAAGAAGGAGAAGGAA
GAAGAGGAGCTGTTTCCCGAGTCAGAGCGGCCAGAGATGCTGAGCGAGCAGGAGCACATG
AGCATCTCGGGCAGTAGCGCCCGACACATGGTGATGCAGAAGCTGCTCCGCAAGCAGGAG
TCTACAGTGATGGTTCTGCGCAACATGGTGGACCCCAAGGACATCGATGATGACCTGGAA
GGGGAGGTGACAGAGGAGTGTGGCAAGTTCGGGGCCGTGAACCGCGTCATCATCTACCAA
GAGAAACAAGGCGAGGAGGAGGATGCAGAAATCATTGTCAAGATCTTTGTGGAGTTTTCC
ATAGCCTCTGAGACTCATAAGGCCATCCAGGCCCTCAATGGCCGCTGGTTTGCTGGCCGC
AAGGTGGTGGCTGAAGTGTACGACCAGGAGCGTTTTGATAACAGTGACCTCTCTGCGTGA

Protein Properties

Number of Residues

559

Molecular Weight

59875.0

Theoretical pI

4.95

Pfam Domain Function

RRM_1 (PF00076 )

Signals

["None"]

Transmembrane Regions

["None"]

Protein Sequence

>Poly(U)-binding-splicing factor PUF60
MATATIALQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLT
PEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQ
RQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVP
EAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDD
DIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVG
KAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTL
AQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKE
EEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLE
GEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGR
KVVAEVYDQERFDNSDLSA

External Links

GenBank ID Protein

6684440

UniProtKB/Swiss-Prot ID

Q9UHX1

UniProtKB/Swiss-Prot Entry Name

PUF60_HUMAN

PDB IDs

Not Available

GenBank Gene ID

AF114818

GeneCard ID