Identification
HMDB Protein ID CDBP04545
Secondary Accession Numbers Not Available
Name E3 ubiquitin-protein ligase RFWD3
Description Not Available
Synonyms
  1. RING finger and WD repeat domain-containing protein 3
  2. RING finger protein 201
Gene Name RFWD3
Protein Type Enzyme
Biological Properties
General Function Involved in protein binding
Specific Function E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53
GO Classification
Function
ion binding
cation binding
metal ion binding
binding
transition metal ion binding
zinc ion binding
protein binding
Cellular Location
  1. Nucleus
Pathways
Gene Properties
Chromosome Location Chromosome:1
Locus 16q23.1
SNPs RFWD3
Gene Sequence
>2325 bp
ATGGCTCATGAAGCAATGGAATATGATGTTCAGGTGCAGTTAAATCATGCCGAACAACAG
CCAGCTCCTGCTGGCATGGCCAGCAGCCAAGGGGGACCAGCCCTCCTCCAGCCTGTTCCT
GCTGATGTGGTCAGCAGCCAGGGGGTACCATCCATCCTCCAGCCAGCTCCTGCTGAGGTG
ATCAGCAGCCAAGCGACACCACCCCTGCTCCAGCCTGCTCCGCAACTGTCTGTTGACCTG
ACAGAAGTGGAGGTCTTGGGAGAAGACACTGTGGAGAACATCAATCCAAGAACTTCAGAA
CAACATAGGCAGGGATCTGATGGTAATCACACCATCCCAGCATCTTCGTTGCATTCAATG
ACCAACTTCATCAGCGGACTGCAGAGACTTCATGGCATGCTGGAATTCCTGAGACCTTCA
TCTTCAAACCACAGTGTAGGGCCAATGAGAACAAGAAGGAGGGTATCTGCTTCACGGAGG
GCAAGAGCCGGAGGGTCTCAGAGGACAGACAGTGCCAGGTTGAGAGCACCATTGGATGCT
TACTTTCAGGTGAGCAGGACCCAGCCTGACTTGCCAGCTACCACTTATGATTCAGAGACT
AGGAATCCTGTATCTGAAGAGTTGCAGGTGTCTAGTAGTTCTGATTCTGACAGTGACAGC
TCTGCAGAGTATGGAGGGGTTGTTGACCAGGCAGAGGAATCTGGAGCTGTCATTTTAGAA
GAGCAACTAGCAGGTGTCTCAGCAGAGCAAGAAGTTACATGTATCGATGGAGGCAAGACC
CTCCCCAAACAGCCATCTCCCCAGAAGTCTGAGCCTCTGCTACCTTCTGCTTCTATGGAT
GAGGAAGAAGGGGACACTTGTACAATATGTCTGGAACAGTGGACCAATGCTGGGGACCAC
CGGCTCTCAGCATTACGCTGTGGGCATCTCTTTGGGTATAGGTGCATTTCCACGTGGCTT
AAAGGACAAGTACGAAAATGTCCCCAGTGCAACAAGAAAGCCAGGCACAGTGACATTGTC
GTCCTTTATGCCCGAACCCTGAGAGCTTTGGACACTAGTGAACAGGAGCGCATGAAAAGT
TCCCTACTGAAGGAACAGATGCTAAGGAAACAGGCCGAGTTAGAATCAGCACAGTGCCGA
CTCCAACTGCAGGTCCTCACTGATAAGTGCACTAGGCTTCAAAGGCGTGTTCAGGACTTG
CAAAAACTTACGTCACATCAAAGTCAGAATTTACAGCAACCCAGGGGCTCCCAAGCATGG
GTCCTGAGCTGCTCACCCTCCAGCCAGGGCCAGCACAAGCACAAGTACCACTTCCAAAAG
ACCTTCACAGTATCTCAGGCAGGAAACTGCCGGATCATGGCATACTGTGATGCTCTGAGC
TGCCTGGTGATATCACAGCCTTCTCCTCAGGCCTCTTTTCTTCCAGGCTTTGGTGTTAAG
ATGTTGAGTACTGCCAACATGAAGAGCAGTCAGTACATTCCGATGCATGGCAAACAGATC
CGTGGACTGGCGTTTAGCAGTTACCTCAGAGGCTTGCTACTCTCTGCTTCCCTAGACAAC
ACTATTAAACTGACCAGCCTGGAGACAAATACCGTGGTCCAGACTTATAATGCTGGACGT
CCTGTCTGGAGCTGTTGCTGGTGTCTTGATGAGGCTAACTACATCTATGCTGGACTGGCC
AATGGTTCAATTCTGGTATATGACGTGCGAAACACGAGCAGTCATGTGCAGGAGTTAGTA
GCTCAGAAAGCCAGATGCCCACTGGTCTCCCTGTCATACATGCCCAGAGCTGCCTCAGCT
GCATTTCCATATGGTGGGGTGCTGGCTGGAACCTTGGAGGATGCTTCATTCTGGGAACAG
AAAATGGACTTTTCTCATTGGCCTCATGTGCTGCCCTTGGAGCCAGGGGGCTGCATAGAC
TTTCAGACAGAGAACAGCTCCCGGCACTGTCTTGTGACCTACAGGCCTGATAAAAATCAC
ACCACCATACGAAGTGTGCTGATGGAAATGTCCTACCGACTGGATGACACTGGAAATCCA
ATCTGCTCCTGCCAGCCTGTACATACATTTTTTGGAGGACCTACTTGCAAACTATTGACC
AAAAATGCCATTTTCCAAAGCCCAGAGAATGATGGCAACATCCTGGTGTGTACTGGGGAT
GAAGCAGCAAATTCTGCCCTGCTGTGGGATGCTGCCAGTGGCTCGTTGCTCCAGGACCTA
CAGACCGATCAGCCTGTGTTGGACATCTGCCCATTTGAGGTGAACCGTAACAGCTACTTG
GCTACCTTAACAGAGAAGATGGTCCACATCTATAAGTGGGAGTGA
Protein Properties
Number of Residues 774
Molecular Weight 85093.1
Theoretical pI 6.43
Pfam Domain Function
Signals
  • ["None"]
Transmembrane Regions
  • ["None"]
Protein Sequence
>E3 ubiquitin-protein ligase RFWD3
MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEV
ISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSM
TNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDA
YFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILE
EQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDH
RLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKS
SLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAW
VLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVK
MLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGR
PVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASA
AFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNH
TTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGD
EAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE
GenBank ID Protein 71143112
UniProtKB/Swiss-Prot ID Q6PCD5
UniProtKB/Swiss-Prot Entry Name RFWD3_HUMAN
PDB IDs Not Available
GenBank Gene ID NM_018124.3
GeneCard ID RFWD3
GenAtlas ID RFWD3
HGNC ID HGNC:25539
References
General References Not Available