| Identification |
|---|
| HMDB Protein ID
| CDBP04480 |
| Secondary Accession Numbers
| Not Available |
| Name
| Hyaluronidase PH-20 |
| Description
| Not Available |
| Synonyms
|
- SubName: Sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding), isoform CRA_c
- Hyal-PH20
- Hyaluronoglucosaminidase PH-20
- Sperm adhesion molecule 1
- Sperm surface protein PH-20
|
| Gene Name
| SPAM1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in catalytic activity |
| Specific Function
| Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
|
| GO Classification
|
| Biological Process |
| carbohydrate metabolic process |
| binding of sperm to zona pellucida |
| fusion of sperm to egg plasma membrane |
| cell adhesion |
| Cellular Component |
| anchored to membrane |
| plasma membrane |
| Function |
| hyalurononglucosaminidase activity |
| hexosaminidase activity |
| catalytic activity |
| hydrolase activity |
| hydrolase activity, acting on glycosyl bonds |
| hydrolase activity, hydrolyzing o-glycosyl compounds |
| Molecular Function |
| hyalurononglucosaminidase activity |
| Process |
| carbohydrate metabolic process |
| cellular process |
| cellular process involved in reproduction |
| cellular process involved in reproduction in multicellular organism |
| fusion of sperm to egg plasma membrane |
| metabolic process |
| primary metabolic process |
|
| Cellular Location
|
- Cytoplasmic
|
| Pathways
|
| Name | SMPDB/Pathwhiz | KEGG | | Glycosaminoglycan degradation | Not Available |  |
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| SPAM1 |
| Gene Sequence
|
>1536 bp
ATGGGAGTGCTAAAATTCAAGCACATCTTTTTCAGAAGCTTTGTTAAATCAAGTGGAGTA
TCCCAGATAGTTTTCACCTTCCTTCTGATTCCATGTTGCTTGACTCTGAATTTCAGAGCA
CCTCCTGTTATTCCAAATGTGCCTTTCCTCTGGGCCTGGAATGCCCCAAGTGAATTTTGT
CTTGGAAAATTTGATGAGCCACTAGATATGAGCCTCTTCTCTTTCATAGGAAGCCCCCGA
ATAAACGCCACCGGGCAAGGTGTTACAATATTTTATGTTGATAGACTTGGCTACTATCCT
TACATAGATTCAATCACAGGAGTAACTGTGAATGGAGGAATCCCCCAGAAGATTTCCTTA
CAAGACCATCTGGACAAAGCTAAGAAAGACATTACATTTTATATGCCAGTAGACAATTTG
GGAATGGCTGTTATTGACTGGGAAGAATGGAGACCCACTTGGGCAAGAAACTGGAAACCT
AAAGATGTTTACAAGAATAGGTCTATTGAATTGGTTCAGCAACAAAATGTACAACTTAGT
CTCACAGAGGCCACTGAGAAAGCAAAACAAGAATTTGAAAAAGCAGGGAAGGATTTCCTG
GTAGAGACTATAAAATTGGGAAAATTACTTCGGCCAAATCACTTGTGGGGTTATTATCTT
TTTCCGGATTGTTACAACCATCACTATAAGAAACCCGGTTACAATGGAAGTTGCTTCAAT
GTAGAAATAAAAAGAAATGATGATCTCAGCTGGTTGTGGAATGAAAGCACTGCTCTTTAC
CCATCCATTTATTTGAACACTCAGCAGTCTCCTGTAGCTGCTACACTCTATGTGCGCAAT
CGAGTTCGGGAAGCCATCAGAGTTTCCAAAATACCTGATGCAAAAAGTCCACTTCCGGTT
TTTGCATATACCCGCATAGTTTTTACTGATCAAGTTTTGAAATTCCTTTCTCAAGATGAA
CTTGTGTATACATTTGGCGAAACTGTTGCTCTGGGTGCTTCTGGAATTGTAATATGGGGA
ACCCTCAGTATAATGCGAAGTATGAAATCTTGCTTGCTCCTAGACAATTACATGGAGACT
ATACTGAATCCTTACATAATCAACGTCACACTAGCAGCCAAAATGTGTAGCCAAGTGCTT
TGCCAGGAGCAAGGAGTGTGTATAAGGAAAAACTGGAATTCAAGTGACTATCTTCACCTC
AACCCAGATAATTTTGCTATTCAACTTGAGAAAGGTGGAAAGTTCACAGTACGTGGAAAA
CCGACACTTGAAGACCTGGAGCAATTTTCTGAAAAATTTTATTGCAGCTGTTATAGCACC
TTGAGTTGTAAGGAGAAAGCTGATGTAAAAGACACTGATGCTGTTGATGTGTGTATTGCT
GATGGTGTCTGTATAGATGCTTTTCTAAAACCTCCCATGGAGACAGAAGAACCTCAAATT
TTCTACAATGCTTCACCCTCCACACTATCTGCCACAATGTTCATTTGGAGGCTGGAAGTC
TGGGATCAAGGTATTAGCAGAATTGGTTTCTTCTGA
|
| Protein Properties |
|---|
| Number of Residues
| 511 |
| Molecular Weight
| Not Available |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFC
LGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISL
QDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLS
LTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFN
VEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPV
FAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMET
ILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGK
PTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQI
FYNASPSTLSATMFIWRLEVWDQGISRIGFF
|
| External Links |
|---|
| GenBank ID Protein
| 20072878 |
| UniProtKB/Swiss-Prot ID
| P38567 |
| UniProtKB/Swiss-Prot Entry Name
| Q8TC30_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC026163 |
| GeneCard ID
| SPAM1 |
| GenAtlas ID
| SPAM1 |
| HGNC ID
| HGNC:11217 |
| References |
|---|
| General References
| Not Available |