| Identification |
|---|
| HMDB Protein ID
| CDBP04457 |
| Secondary Accession Numbers
| Not Available |
| Name
| E3 ubiquitin-protein ligase RNF8 |
| Description
| Not Available |
| Synonyms
|
- RING finger protein 8
|
| Gene Name
| RNF8 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in acid-amino acid ligase activity |
| Specific Function
| E3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'- linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBE2N/UBC13. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBE2N but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys- 63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2 |
| GO Classification
|
| Component |
| organelle |
| membrane-bounded organelle |
| intracellular membrane-bounded organelle |
| nucleus |
| Function |
| ion binding |
| cation binding |
| metal ion binding |
| binding |
| catalytic activity |
| transition metal ion binding |
| zinc ion binding |
| ligase activity |
| protein binding |
| ligase activity, forming carbon-nitrogen bonds |
| acid-amino acid ligase activity |
| Process |
| response to stimulus |
| response to stress |
| metabolic process |
| macromolecule metabolic process |
| cellular process |
| protein modification by small protein conjugation or removal |
| protein modification by small protein conjugation |
| cell division |
| protein ubiquitination |
| macromolecule modification |
| protein modification process |
| cellular response to stress |
| response to dna damage stimulus |
|
| Cellular Location
|
- Nucleus
- Midbody
|
| Pathways
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Chromosome:6 |
| Locus
| 6p21.3 |
| SNPs
| RNF8 |
| Gene Sequence
|
>1458 bp
ATGGGGGAGCCCGGCTTCTTCGTCACAGGAGACCGCGCCGGTGGCCGGAGCTGGTGCCTG
CGGCGGGTGGGGATGAGCGCCGGGTGGCTGCTGCTGGAAGATGGGTGCGAGGTGACTGTA
GGACGAGGATTTGGTGTCACATACCAACTGGTATCAAAAATCTGCCCCCTGATGATTTCT
CGAAACCACTGTGTTTTGAAGCAGAATCCTGAGGGCCAATGGACAATTATGGACAACAAG
AGTCTAAATGGTGTTTGGCTGAACAGAGCGCGTCTGGAACCTTTAAGGGTCTATTCCATT
CATCAGGGAGACTACATCCAACTTGGAGTGCCTCTGGAAAATAAGGAGAATGCGGAGTAT
GAATATGAAGTTACTGAAGAAGACTGGGAGACAATATATCCTTGTCTTTCCCCAAAGAAT
GACCAAATGATAGAAAAAAATAAGGAATTGAGAACTAAAAGGAAATTCAGTTTGGATGAA
TTAGCAGGTCCTGGAGCTGAAGGCCCCTCAAATTTGAAATCCAAAATAAATAAAGTGTCT
TGTGAATCTGGTCAGCCAGTGAAATCACAGGGGAAAGGTGAAGTGGCCAGTACACCCTCT
GACAATTTGGATCCTAAGTTGACTGCCCTTGAGCCAAGTAAGACCACAGGGGCTCCCATT
TACCCTGGCTTCCCCAAAGTCACAGAGGTTCATCATGAGCAGAAAGCCTCAAACTCTTCA
GCATCTCAGAGAAGCTTACAGATGTTTAAGGTGACCATGTCCAGGATTCTGAGGCTCAAA
ATACAGATGCAGGAAAAACATGAAGCCGTTATGAATGTGAAAAAGCAGACCCAAAAGGGG
AACTCAAAGAAAGTTGTGCAAATGGAGCAGGAACTTCAGGACTTACAGTCCCAGCTGTGT
GCAGAGCAGGCTCAGCAGCAGGCAAGAGTGGAGCAACTAGAGAAGACTTTCCAGGAAGAG
GAACAGCATCTTCAGGGTTTGGAGATAGCCCAAGGAGAAAAGGACCTGAAGCAACAGCTG
GCCCAGGCTCTGCAGGAGCATTGGGCTCTAATGGAAGAGCTAAATCGCAGCAAGAAGGAC
TTTGAAGCAATCATTCAAGCCAAGAACAAAGAATTAGAGCAGACCAAGGAAGAGAAGGAG
AAGATGCAAGCACAGAAGGAAGAAGTTCTTAGCCACATGAATGATGTGCTAGAGAATGAG
CTCCAATGTATTATTTGTTCAGAATACTTCATTGAGGCTGTCACCTTGAACTGTGCCCAC
AGTTTCTGCTCCTACTGTATCAATGAATGGATGAAGCGGAAGATAGAATGCCCCATTTGT
CGGAAGGACATTAAGTCCAAAACGTACTCTTTGGTTCTGGACAATTGCATTAATAAGATG
GTAAATAATCTGAGCTCAGAAGTGAAAGAACGACGAATTGTTCTCATTAGGGAACGAAAA
GCAAAGAGATTGTTCTGA
|
| Protein Properties |
|---|
| Number of Residues
| 485 |
| Molecular Weight
| 55517.2 |
| Theoretical pI
| 7.37 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>E3 ubiquitin-protein ligase RNF8
MGEPGFFVTGDRAGGRSWCLRRVGMSAGWLLLEDGCEVTVGRGFGVTYQLVSKICPLMIS
RNHCVLKQNPEGQWTIMDNKSLNGVWLNRARLEPLRVYSIHQGDYIQLGVPLENKENAEY
EYEVTEEDWETIYPCLSPKNDQMIEKNKELRTKRKFSLDELAGPGAEGPSNLKSKINKVS
CESGQPVKSQGKGEVASTPSDNLDPKLTALEPSKTTGAPIYPGFPKVTEVHHEQKASNSS
ASQRSLQMFKVTMSRILRLKIQMQEKHEAVMNVKKQTQKGNSKKVVQMEQELQDLQSQLC
AEQAQQQARVEQLEKTFQEEEQHLQGLEIAQGEKDLKQQLAQALQEHWALMEELNRSKKD
FEAIIQAKNKELEQTKEEKEKMQAQKEEVLSHMNDVLENELQCIICSEYFIEAVTLNCAH
SFCSYCINEWMKRKIECPICRKDIKSKTYSLVLDNCINKMVNNLSSEVKERRIVLIRERK
AKRLF
|
| External Links |
|---|
| GenBank ID Protein
| 3721580 |
| UniProtKB/Swiss-Prot ID
| O76064 |
| UniProtKB/Swiss-Prot Entry Name
| RNF8_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| AB012770 |
| GeneCard ID
| RNF8 |
| GenAtlas ID
| RNF8 |
| HGNC ID
| HGNC:10071 |
| References |
|---|
| General References
| Not Available |