| Identification |
|---|
| HMDB Protein ID
| CDBP04429 |
| Secondary Accession Numbers
| Not Available |
| Name
| E3 ubiquitin-protein ligase CHFR |
| Description
| Not Available |
| Synonyms
|
- Checkpoint with forkhead and RING finger domains protein
- RING finger protein 196
|
| Gene Name
| CHFR |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in protein binding |
| Specific Function
| E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress |
| GO Classification
|
| Function |
| zinc ion binding |
| protein binding |
| ion binding |
| cation binding |
| metal ion binding |
| binding |
| transition metal ion binding |
|
| Cellular Location
|
- Nucleus
- PML body
|
| Pathways
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Chromosome:1 |
| Locus
| 12q24.33 |
| SNPs
| CHFR |
| Gene Sequence
|
>1995 bp
ATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCCCTGGGGACGGCTCCTG
CGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCCTCCTGAGGAAGCGGGAGTGGACCATC
GGGCGGAGACGAGGTTGCGACCTTTCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCAC
TGTAGAATTGTAGTGGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGT
GGAACAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAGACTGGG
GATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAACGTGGCATACCTCTAT
GAATCTTTAAGTGAAAAGCAAGGCATGACACAAGAATCCTTTGAAGCTAACAAGGAAAAT
GTGTTCCATGGGACCAAAGATACCTCAGGTGCAGGTGCAGGGCGAGGGGCCGATCCCCGG
GTCCCTCCGTCGTCGCCCGCCACTCAGGTGTGCTTTGAGGAACCACAGCCATCAACATCG
ACGTCAGACCTCTTCCCCACAGCCTCGGCCTCTTCCACGGAGCCTTCTCCTGCAGGGCGA
GAGCGTTCCTCCAGTTGTGGGTCTGGGGGTGGTGGCATCTCCCCTAAAGGAAGTGGTCCC
TCTGTGGCAAGTGATGAAGTCTCCAGCTTTGCCTCAGCTCTCCCAGACAGAAAGACTGCG
TCCTTTTCGTCGTTGGAACCCCAGGATCAGGAGGATTTGGAGCCCGTGAAGAAGAAAATG
AGAGGAGATGGGGACCTTGACCTGAACGGGCAGTTGTTGGTCGCACAACCGCGTAGAAAT
GCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGAAGCCAGACAAGATGGAGGAG
ACGCTGACATGCATCATCTGCCAGGACCTGCTGCACGACTGCGTGAGTTTGCAGCCCTGC
ATGCACACGTTCTGCGCGGCTTGCTACTCGGGCTGGATGGAGCGCTCGTCCCTGTGTCCT
ACCTGCCGCTGTCCCGTGGAGCGGATCTGTAAAAACCACATCCTCAACAACCTCGTGGAA
GCATACCTCATCCAGCATCCAGACAAGAGTCGCAGTGAAGAAGATGTGCAAAGTATGGAT
GCCAGGAATAAAATCACTCAAGACATGCTGCAGCCCAAAGTCAGGCGGTCTTTTTCTGAT
GAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCAGACGTTGACAGTGAGTCCTCAGAC
ATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTCCTGAGTACAGAAGGCAGGCGGCGCAG
CCTCCCCACTGCCCAGCACCCGAGGGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCA
CCCTCCACGTCCGTCAGCCTGACGACAGCAGTCCAGGATTACGTGTGCCCTCTGCAAGGA
AGCCACGCCCTGTGCACCTGCTGCTTCCAGCCCATGCCCGACCGGAGAGCGGAGCGCGAG
CAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCGGTCTGCCTGCAGCCTTTCTGCCACCTG
TACTGGGGCTGCACCCGGACCGGCTGCTACGGCTGCCTGGCCCCGTTTTGTGAGCTCAAC
CTGGGTGACAAGTGTCTGGACGGCGTGCTGAACAACAACAGCTACGAGTCAGACATCCTG
AAGAATTACCTGGCAACCAGAGGTTTGACATGGAAAAACATGTTGACCGAGAGCCTCGTG
GCTCTCCAGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCACGGGAGACACCGTTCTG
TGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTGACCTATCAGTATCGGCAGAACATT
CCTGCTTCCGAGTTGCCAGTGGCCGTAACATCCCGTCCTGACTGCTACTGGGGCCGTAAC
TGCCGCACTCAGGTGAAAGCTCACCACGCCATGAAATTCAATCATATCTGTGAACAGACA
AGGTTCAAAAACTAA
|
| Protein Properties |
|---|
| Number of Residues
| 664 |
| Molecular Weight
| 73385.8 |
| Theoretical pI
| 5.9 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>E3 ubiquitin-protein ligase CHFR
MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDH
CRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLY
ESLSEKQGMTQESFEANKENVFHGTKDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTS
TSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTA
SFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEE
TLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVE
AYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSD
ISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQG
SHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELN
LGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVL
CYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQT
RFKN
|
| External Links |
|---|
| GenBank ID Protein
| 239048792 |
| UniProtKB/Swiss-Prot ID
| Q96EP1 |
| UniProtKB/Swiss-Prot Entry Name
| CHFR_HUMAN |
| PDB IDs
|
|
| GenBank Gene ID
| NM_001161344.1 |
| GeneCard ID
| CHFR |
| GenAtlas ID
| CHFR |
| HGNC ID
| HGNC:20455 |
| References |
|---|
| General References
| Not Available |