| Identification |
|---|
| HMDB Protein ID
| CDBP04319 |
| Secondary Accession Numbers
| Not Available |
| Name
| Alkaline ceramidase 2 |
| Description
| Not Available |
| Synonyms
|
- Acylsphingosine deacylase 3-like
- AlkCDase 2
- Alkaline CDase 2
- N-acylsphingosine amidohydrolase 3-like
- haCER2
|
| Gene Name
| ACER2 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| Specific Function
| Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. The substrate preference is D-erythro-C(18:1)-, C(20:1)-, C(20:4)-ceramide > D-erythro-C(16:0)-, C(18:0), C(20:0)-ceramide > D-erythro-C(24:1)-ceramide > D-erythro-C(12:0)-ceramide, D-erythro-C(14:0)-ceramides > D-erythro-C(24:0)-ceramide > D-erythro-C(6:0)-ceramide. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate.
|
| GO Classification
|
| Biological Process |
| phospholipid metabolic process |
| sphingosine biosynthetic process |
| activation of cysteine-type endopeptidase activity involved in apoptotic process |
| cellular response to drug |
| ceramide metabolic process |
| negative regulation of cell adhesion mediated by integrin |
| negative regulation of cell-matrix adhesion |
| negative regulation of protein glycosylation in Golgi |
| positive regulation of cell death |
| positive regulation of cell proliferation |
| response to retinoic acid |
| Cellular Component |
| integral to Golgi membrane |
| Component |
| intrinsic to membrane |
| integral to membrane |
| cell part |
| membrane part |
| Function |
| catalytic activity |
| hydrolase activity |
| hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds |
| hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| Molecular Function |
| dihydroceramidase activity |
| Process |
| sphingoid metabolic process |
| metabolic process |
| ceramide metabolic process |
| sphingolipid metabolic process |
| membrane lipid metabolic process |
| primary metabolic process |
| lipid metabolic process |
| cellular lipid metabolic process |
|
| Cellular Location
|
- Golgi apparatus membrane
- Multi-pass membrane protein
|
| Pathways
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 9 |
| Locus
| 9p22.1 |
| SNPs
| ACER2 |
| Gene Sequence
|
>828 bp
ATGGGCGCCCCGCACTGGTGGGACCAGCTGCAGGCTGGTAGCTCGGAGGTGGACTGGTGC
GAGGACAACTACACCATCGTGCCTGCTATCGCCGAGTTCTACAACACGATCAGCAATGTC
TTATTTTTCATTTTACCGCCCATCTGCATGTGCTTGTTTCGTCAGTATGCAACATGCTTC
AACAGTGGCATCTACTTAATCTGGACTCTTTTGGTTGTAGTGGGAATTGGATCCGTCTAC
TTCCATGCAACCCTTAGTTTCTTGGGTCAGATGCTTGATGAACTTGCAGTCCTTTGGGTT
CTGATGTGTGCTTTGGCCATGTGGTTCCCCAGAAGGTATCTACCAAAGATCTTTCGGAAT
GACCGGGGTAGGTTCAAGGTGGTGGTCAGTGTCCTGTCTGCGGTTACGACGTGCCTGGCA
TTTGTCAAGCCTGCCATCAACAACATCTCTCTGATGACCCTGGGAGTTCCTTGCACTGCA
CTGCTCATCGCAGAGCTAAAGAGGTGTGACAACATGCGTGTGTTTAAGCTGGGCCTCTTC
TCGGGCCTCTGGTGGACCCTGGCCCTGTTCTGCTGGATCAGTGACCGAGCTTTCTGCGAG
CTGCTGTCATCCTTCAACTTCCCCTACCTGCACTGCATGTGGCACATCCTCATCTGCCTT
GCTGCCTACCTGGGCTGTGTATGCTTTGCCTACTTTGATGCTGCCTCAGAGATTCCTGAG
CAAGGCCCTGTCATCAAGTTCTGGCCCAATGAGAAATGGGCCTTCATTGGTGTCCCCTAT
GTGTCCCTCCTGTGTGCCAACAAGAAATCATCAGTCAAGACCACGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 275 |
| Molecular Weight
| 31308.85 |
| Theoretical pI
| 7.654 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Alkaline ceramidase 2
MGAPHWWDQLQAGSSEVDWCEDNYTIVPAIAEFYNTISNVLFFILPPICMCLFRQYATCF
NSGIYLIWTLLVVVGIGSVYFHATLSFLGQMLDELAVLWVLMCALAMWFPRRYLPKIFRN
DRGRFKVVVSVLSAVTTCLAFVKPAINNISLMTLGVPCTALLIAELKRCDNMRVFKLGLF
SGLWWTLALFCWISDRAFCELLSSFNFPYLHCMWHILICLAAYLGCVCFAYFDAASEIPE
QGPVIKFWPNEKWAFIGVPYVSLLCANKKSSVKIT
|
| External Links |
|---|
| GenBank ID Protein
| 36304156 |
| UniProtKB/Swiss-Prot ID
| Q5QJU3 |
| UniProtKB/Swiss-Prot Entry Name
| ACER2_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| AY312516 |
| GeneCard ID
| ACER2 |
| GenAtlas ID
| ACER2 |
| HGNC ID
| HGNC:23675 |
| References |
|---|
| General References
| Not Available |