| Identification |
|---|
| HMDB Protein ID
| CDBP04043 |
| Secondary Accession Numbers
| Not Available |
| Name
| Werner syndrome ATP-dependent helicase |
| Description
| Not Available |
| Synonyms
|
- DNA helicase, RecQ-like type 3
- Exonuclease WRN
- RecQ protein-like 2
- RecQ3
|
| Gene Name
| WRN |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in nucleic acid binding |
| Specific Function
| Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).
|
| GO Classification
|
| Biological Process |
| response to UV-C |
| nucleolus to nucleoplasm transport |
| positive regulation of hydrolase activity |
| replicative cell aging |
| DNA recombination |
| multicellular organismal aging |
| response to oxidative stress |
| replication fork processing |
| telomere maintenance |
| DNA synthesis involved in DNA repair |
| cell aging |
| base-excision repair |
| regulation of apoptotic process |
| cellular response to starvation |
| regulation of growth rate |
| Cellular Component |
| centrosome |
| nucleolus |
| nucleoplasm |
| Component |
| cell part |
| intracellular |
| Function |
| adenyl nucleotide binding |
| hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides |
| pyrophosphatase activity |
| adenyl ribonucleotide binding |
| atp binding |
| atp-dependent helicase activity |
| dna helicase activity |
| atp-dependent dna helicase activity |
| exonuclease activity |
| 3'-5' exonuclease activity |
| atpase activity |
| atpase activity, coupled |
| hydrolase activity, acting on ester bonds |
| nucleic acid binding |
| binding |
| atp-dependent 3'-5' dna helicase activity |
| catalytic activity |
| nucleoside-triphosphatase activity |
| hydrolase activity |
| helicase activity |
| nucleoside binding |
| nuclease activity |
| purine nucleoside binding |
| hydrolase activity, acting on acid anhydrides |
| Molecular Function |
| ATP-dependent 3'-5' DNA helicase activity |
| manganese ion binding |
| protein complex binding |
| magnesium ion binding |
| Y-form DNA binding |
| ATP binding |
| ATP-dependent DNA helicase activity |
| bubble DNA binding |
| four-way junction helicase activity |
| G-quadruplex DNA binding |
| 3'-5' exonuclease activity |
| 3'-5' DNA helicase activity |
| protein homodimerization activity |
| Process |
| metabolic process |
| nitrogen compound metabolic process |
| cellular nitrogen compound metabolic process |
| nucleobase, nucleoside, nucleotide and nucleic acid metabolic process |
| macromolecule metabolic process |
| cellular macromolecule metabolic process |
| dna metabolic process |
| dna replication |
| dna recombination |
| dna repair |
|
| Cellular Location
|
- Nucleus
- Nucleus
- nucleolus
|
| Pathways
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 8 |
| Locus
| 8p12 |
| SNPs
| WRN |
| Gene Sequence
|
>4299 bp
ATGAGTGAAAAAAAATTGGAAACAACTGCACAGCAGCGGAAATGTCCTGAATGGATGAAT
GTGCAGAATAAAAGATGTGCTGTAGAAGAAAGAAAGGCATGTGTTCGGAAGAGTGTTTTT
GAAGATGACCTCCCCTTCTTAGAATTCACTGGATCCATTGTGTATAGTTACGATGCTAGT
GATTGCTCTTTCCTGTCAGAAGATATTAGCATGAGTCTATCAGATGGGGATGTGGTGGGA
TTTGACATGGAGTGGCCACCATTATACAATAGAGGGAAACTTGGCAAAGTTGCACTAATT
CAGTTGTGTGTTTCTGAGAGCAAATGTTACTTGTTCCACGTTTCTTCCATGTCAGTTTTT
CCCCAGGGATTAAAAATGTTGCTTGAAAATAAAGCAGTTAAAAAGGCAGGTGTAGGAATT
GAAGGAGATCAGTGGAAACTTCTACGTGACTTTGATATCAAATTGAAGAATTTTGTGGAG
TTGACAGATGTTGCCAATAAAAAGCTGAAATGTACAGAGACCTGGAGCCTTAACAGTCTG
GTTAAACACCTCTTAGGTAAACAGCTCCTGAAAGACAAGTCTATCCGCTGTAGCAATTGG
AGTAAATTTCCTCTCACTGAGGACCAGAAACTGTATGCAGCCACTGATGCTTATGCTGGT
TTTATTATTTACCGAAATTTAGAGATTTTGGATGATACTGTGCAAAGGTTTGCTATAAAT
AAAGAGGAAGAAATCCTACTTAGCGACATGAACAAACAGTTGACTTCAATCTCTGAGGAA
GTGATGGATCTGGCTAAGCATCTTCCTCATGCTTTCAGTAAATTGGAAAACCCACGGAGG
GTTTCTATCTTACTAAAGGATATTTCAGAAAATCTATATTCACTGAGGAGGATGATAATT
GGGTCTACTAACATTGAGACTGAACTGAGGCCCAGCAATAATTTAAACTTATTATCCTTT
GAAGATTCAACTACTGGGGGAGTACAACAGAAACAAATTAGAGAACATGAAGTTTTAATT
CACGTTGAAGATGAAACATGGGACCCAACACTTGATCATTTAGCTAAACATGATGGAGAA
GATGTACTTGGAAATAAAGTGGAACGAAAAGAAGATGGATTTGAAGATGGAGTAGAAGAC
AACAAATTGAAAGAGAATATGGAAAGAGCTTGTTTGATGTCGTTAGATATTACAGAACAT
GAACTCCAAATTTTGGAACAGCAGTCTCAGGAAGAATATCTTAGTGATATTGCTTATAAA
TCTACTGAGCATTTATCTCCCAATGATAATGAAAACGATACGTCCTATGTAATTGAGAGT
GATGAAGATTTAGAAATGGAGATGCTTAAGCATTTATCTCCCAATGATAATGAAAACGAT
ACGTCCTATGTAATTGAGAGTGATGAAGATTTAGAAATGGAGATGCTTAAGTCTTTAGAA
AACCTCAATAGTGGCACGGTAGAACCAACTCATTCTAAATGCTTAAAAATGGAAAGAAAT
CTGGGTCTTCCTACTAAAGAAGAAGAAGAAGATGATGAAAATGAAGCTAATGAAGGGGAA
GAAGATGATGATAAGGACTTTTTGTGGCCAGCACCCAATGAAGAGCAAGTTACTTGCCTC
AAGATGTACTTTGGCCATTCCAGTTTTAAACCAGTTCAGTGGAAAGTGATTCATTCAGTA
TTAGAAGAAAGAAGAGATAATGTTGCTGTCATGGCAACTGGATATGGAAAGAGTTTGTGC
TTCCAGTATCCACCTGTTTATGTAGGCAAGATTGGCCTTGTTATCTCTCCCCTTATTTCT
CTGATGGAAGACCAAGTGCTACAGCTTAAAATGTCCAACATCCCAGCTTGCTTCCTTGGA
TCAGCACAGTCAGAAAATGTTCTAACAGATATTAAATTAGGTAAATACCGGATTGTATAC
GTAACTCCAGAATACTGTTCAGGTAACATGGGCCTGCTCCAGCAACTTGAGGCTGATATT
GGTATCACGCTCATTGCTGTGGATGAGGCTCACTGTATTTCTGAGTGGGGGCATGATTTT
AGGGATTCATTCAGGAAGTTGGGCTCCCTAAAGACAGCACTGCCAATGGTTCCAATCGTT
GCACTTACTGCTACTGCAAGTTCTTCAATCCGGGAAGACATTGTACGTTGCTTAAATCTG
AGAAATCCTCAGATCACCTGTACTGGTTTTGATCGACCAAACCTGTATTTAGAAGTTAGG
CGAAAAACAGGGAATATCCTTCAGGATCTGCAGCCATTTCTTGTCAAAACAAGTTCCCAC
TGGGAATTTGAAGGTCCAACAATCATCTACTGTCCTTCTAGAAAAATGACACAACAAGTT
ACAGGTGAACTTAGGAAACTTAATCTATCCTGTGGAACATACCATGCGGGCATGAGTTTT
AGCACAAGGAAAGACATTCATCATAGGTTTGTAAGAGATGAAATTCAGTGTGTCATAGCT
ACCATAGCTTTTGGAATGGGCATTAATAAAGCTGACATTCGCCAAGTCATTCATTACGGT
GCTCCTAAGGACATGGAATCATATTATCAGGAGATTGGTAGAGCTGGTCGTGATGGACTT
CAAAGTTCTTGTCACGTCCTCTGGGCTCCTGCAGACATTAACTTAAATAGGCACCTTCTT
ACTGAGATACGTAATGAGAAGTTTCGATTATACAAATTAAAGATGATGGCAAAGATGGAA
AAATATCTTCATTCTAGCAGATGTAGGAGACAAATCATCTTGTCTCATTTTGAGGACAAA
CAAGTACAAAAAGCCTCCTTGGGAATTATGGGAACTGAAAAATGCTGTGATAATTGCAGG
TCCAGATTGGATCATTGCTATTCCATGGATGACTCAGAGGATACATCCTGGGACTTTGGT
CCACAAGCATTTAAGCTTTTGTCTGCTGTGGACATCTTAGGCGAAAAATTTGGAATTGGG
CTTCCAATTTTATTTCTCCGAGGATCTAATTCTCAGCGTCTTGCCGATCAATATCGCAGG
CACAGTTTATTTGGCACTGGCAAGGATCAAACAGAGAGTTGGTGGAAGGCTTTTTCCCGT
CAGCTGATCACTGAGGGATTCTTGGTAGAAGTTTCTCGGTATAACAAATTTATGAAGATT
TGCGCCCTTACGAAAAAGGGTAGAAATTGGCTTCATAAAGCTAATACAGAATCTCAGAGC
CTCATCCTTCAAGCTAATGAAGAATTGTGTCCAAAGAAGTTTCTTCTGCCTAGTTCGAAA
ACTGTATCTTCGGGCACCAAAGAGCATTGTTATAATCAAGTACCAGTTGAATTAAGTACA
GAGAAGAAGTCTAACTTGGAGAAGTTATATTCTTATAAACCATGTGATAAGATTTCTTCT
GGGAGTAACATTTCTAAAAAAAGTATCATGGTACAGTCACCAGAAAAAGCTTACAGTTCC
TCACAGCCTGTTATTTCGGCACAAGAGCAGGAGACTCAGATTGTGTTATATGGCAAATTG
GTAGAAGCTAGGCAGAAACATGCCAATAAAATGGATGTTCCCCCAGCTATTCTGGCAACA
AACAAGATACTGGTGGATATGGCCAAAATGAGACCAACTACGGTTGAAAACGTAAAAAGG
ATTGATGGTGTTTCTGAAGGCAAAGCTGCCATGTTGGCCCCTCTGTTGGAAGTCATCAAA
CATTTCTGCCAAACAAATAGTGTTCAGACAGACCTCTTTTCAAGTACAAAACCTCAAGAA
GAACAGAAGACGAGTCTGGTAGCAAAAAATAAAATATGCACACTTTCACAGTCTATGGCC
ATCACATACTCTTTATTCCAAGAAAAGAAGATGCCTTTGAAGAGCATAGCTGAGAGCAGG
ATTCTGCCTCTCATGACAATTGGCATGCACTTATCCCAAGCGGTGAAAGCTGGCTGCCCC
CTTGATTTGGAGCGAGCAGGCCTGACTCCAGAGGTTCAGAAGATTATTGCTGATGTTATC
CGAAACCCTCCCGTCAACTCAGATATGAGTAAAATTAGCCTAATCAGAATGTTAGTTCCT
GAAAACATTGACACGTACCTTATCCACATGGCAATTGAGATCCTTAAACATGGTCCTGAC
AGCGGACTTCAACCTTCATGTGATGTCAACAAAAGGAGATGTTTTCCCGGTTCTGAAGAG
ATCTGTTCAAGTTCTAAGAGAAGCAAGGAAGAAGTAGGCATCAATACTGAGACTTCATCT
GCAGAGAGAAAGAGACGATTACCTGTGTGGTTTGCCAAAGGAAGTGATACCAGCAAGAAA
TTAATGGACAAAACGAAAAGGGGAGGTCTTTTTAGTTAA
|
| Protein Properties |
|---|
| Number of Residues
| 1432 |
| Molecular Weight
| 162459.405 |
| Theoretical pI
| 6.345 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Werner syndrome ATP-dependent helicase
MSEKKLETTAQQRKCPEWMNVQNKRCAVEERKACVRKSVFEDDLPFLEFTGSIVYSYDAS
DCSFLSEDISMSLSDGDVVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVF
PQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTETWSLNSL
VKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLEILDDTVQRFAIN
KEEEILLSDMNKQLTSISEEVMDLAKHLPHAFSKLENPRRVSILLKDISENLYSLRRMII
GSTNIETELRPSNNLNLLSFEDSTTGGVQQKQIREHEVLIHVEDETWDPTLDHLAKHDGE
DVLGNKVERKEDGFEDGVEDNKLKENMERACLMSLDITEHELQILEQQSQEEYLSDIAYK
STEHLSPNDNENDTSYVIESDEDLEMEMLKHLSPNDNENDTSYVIESDEDLEMEMLKSLE
NLNSGTVEPTHSKCLKMERNLGLPTKEEEEDDENEANEGEEDDDKDFLWPAPNEEQVTCL
KMYFGHSSFKPVQWKVIHSVLEERRDNVAVMATGYGKSLCFQYPPVYVGKIGLVISPLIS
LMEDQVLQLKMSNIPACFLGSAQSENVLTDIKLGKYRIVYVTPEYCSGNMGLLQQLEADI
GITLIAVDEAHCISEWGHDFRDSFRKLGSLKTALPMVPIVALTATASSSIREDIVRCLNL
RNPQITCTGFDRPNLYLEVRRKTGNILQDLQPFLVKTSSHWEFEGPTIIYCPSRKMTQQV
TGELRKLNLSCGTYHAGMSFSTRKDIHHRFVRDEIQCVIATIAFGMGINKADIRQVIHYG
APKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLNRHLLTEIRNEKFRLYKLKMMAKME
KYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCCDNCRSRLDHCYSMDDSEDTSWDFG
PQAFKLLSAVDILGEKFGIGLPILFLRGSNSQRLADQYRRHSLFGTGKDQTESWWKAFSR
QLITEGFLVEVSRYNKFMKICALTKKGRNWLHKANTESQSLILQANEELCPKKFLLPSSK
TVSSGTKEHCYNQVPVELSTEKKSNLEKLYSYKPCDKISSGSNISKKSIMVQSPEKAYSS
SQPVISAQEQETQIVLYGKLVEARQKHANKMDVPPAILATNKILVDMAKMRPTTVENVKR
IDGVSEGKAAMLAPLLEVIKHFCQTNSVQTDLFSSTKPQEEQKTSLVAKNKICTLSQSMA
ITYSLFQEKKMPLKSIAESRILPLMTIGMHLSQAVKAGCPLDLERAGLTPEVQKIIADVI
RNPPVNSDMSKISLIRMLVPENIDTYLIHMAIEILKHGPDSGLQPSCDVNKRRCFPGSEE
ICSSSKRSKEEVGINTETSSAERKRRLPVWFAKGSDTSKKLMDKTKRGGLFS
|
| External Links |
|---|
| GenBank ID Protein
| 3719421 |
| UniProtKB/Swiss-Prot ID
| Q14191 |
| UniProtKB/Swiss-Prot Entry Name
| WRN_HUMAN |
| PDB IDs
|
|
| GenBank Gene ID
| AF091214 |
| GeneCard ID
| WRN |
| GenAtlas ID
| WRN |
| HGNC ID
| HGNC:12791 |
| References |
|---|
| General References
| Not Available |