Identification
HMDB Protein ID CDBP04016
Secondary Accession Numbers Not Available
Name 2-5A-dependent ribonuclease
Description Not Available
Synonyms
  1. 2-5A-dependent RNase
  2. RNase L
  3. Ribonuclease 4
  4. Ribonuclease L
Gene Name RNASEL
Protein Type Enzyme
Biological Properties
General Function Involved in endoribonuclease activity, producing 5'-phosphomonoesters
Specific Function Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress- response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. Might play a central role in the regulation of mRNA. turnover
GO Classification
Function
binding
catalytic activity
endoribonuclease activity, producing 5'-phosphomonoesters
hydrolase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
kinase activity
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
adenyl ribonucleotide binding
atp binding
protein kinase activity
nuclease activity
endonuclease activity
endoribonuclease activity
hydrolase activity, acting on ester bonds
Process
rna metabolic process
phosphorus metabolic process
phosphate metabolic process
metabolic process
macromolecule metabolic process
cellular macromolecule metabolic process
rna processing
cellular metabolic process
protein amino acid phosphorylation
mrna processing
phosphorylation
Cellular Location
  1. Cytoplasm
  2. Mitochondrion
Pathways
Gene Properties
Chromosome Location Chromosome:1
Locus 1q25
SNPs RNASEL
Gene Sequence
>2226 bp
ATGGAGAGCAGGGATCATAACAACCCCCAGGAGGGACCCACGTCCTCCAGCGGTAGAAGG
GCTGCAGTGGAAGACAATCACTTGCTGATTAAAGCTGTTCAAAACGAAGATGTTGACCTG
GTCCAGCAATTGCTGGAAGGTGGAGCCAATGTTAATTTCCAGGAAGAGGAAGGGGGCTGG
ACACCTCTGCATAACGCAGTACAAATGAGCAGGGAGGACATTGTGGAACTTCTGCTTCGT
CATGGTGCTGACCCTGTTCTGAGGAAGAAGAATGGGGCCACGCCTTTTATCCTCGCAGCG
ATTGCGGGGAGCGTGAAGCTGCTGAAACTTTTCCTTTCTAAAGGAGCAGATGTCAATGAG
TGTGATTTTTATGGCTTCACAGCCTTCATGGAAGCCGCTGTGTATGGTAAGGTCAAAGCC
CTAAAATTCCTTTATAAGAGAGGAGCAAATGTGAATTTGAGGCGAAAGACAAAGGAGGAT
CAAGAGCGGCTGAGGAAAGGAGGGGCCACAGCTCTCATGGACGCTGCTGAAAAAGGACAC
GTAGAGGTCTTGAAGATTCTCCTTGATGAGATGGGGGCAGATGTAAACGCCTGTGACAAT
ATGGGCAGAAATGCCTTGATCCATGCTCTCCTGAGCTCTGACGATAGTGATGTGGAGGCT
ATTACGCATCTGCTGCTGGACCATGGGGCTGATGTCAATGTGAGGGGAGAAAGAGGGAAG
ACTCCCCTGATCCTGGCAGTGGAGAAGAAGCACTTGGGTTTGGTGCAGAGGCTTCTGGAG
CAAGAGCACATAGAGATTAATGACACAGACAGTGATGGCAAAACAGCACTGCTGCTTGCT
GTTGAACTCAAACTGAAGAAAATCGCCGAGTTGCTGTGCAAACGTGGAGCCAGTACAGAT
TGTGGGGATCTTGTTATGACAGCGAGGCGGAATTATGACCATTCCCTTGTGAAGGTTCTT
CTCTCTCATGGAGCCAAAGAAGATTTTCACCCTCCTGCTGAAGACTGGAAGCCTCAGAGC
TCACACTGGGGGGCAGCCCTGAAGGATCTCCACAGAATATACCGCCCTATGATTGGCAAA
CTCAAGTTCTTTATTGATGAAAAATACAAAATTGCTGATACTTCAGAAGGAGGCATCTAC
CTGGGGTTCTATGAGAAGCAAGAAGTAGCTGTGAAGACGTTCTGTGAGGGCAGCCCACGT
GCACAGCGGGAAGTCTCTTGTCTGCAAAGCAGCCGAGAGAACAGTCACTTGGTGACATTC
TATGGGAGTGAGAGCCACAGGGGCCACTTGTTTGTGTGTGTCACCCTCTGTGAGCAGACT
CTGGAAGCGTGTTTGGATGTGCACAGAGGGGAAGATGTGGAAAATGAGGAAGATGAATTT
GCCCGAAATGTCCTGTCATCTATATTTAAGGCTGTTCAAGAACTACACTTGTCCTGTGGA
TACACCCACCAGGATCTGCAACCACAAAACATCTTAATAGATTCTAAGAAAGCTGCTCAC
CTGGCAGATTTTGATAAGAGCATCAAGTGGGCTGGAGATCCACAGGAAGTCAAGAGAGAT
CTAGAGGACCTTGGACGGCTGGTCCTCTATGTGGTAAAGAAGGGAAGCATCTCATTTGAG
GATCTGAAAGCTCAAAGTAATGAAGAGGTGGTTCAACTTTCTCCAGATGAGGAAACTAAG
GACCTCATTCATCGTCTCTTCCATCCTGGGGAACATGTGAGGGACTGTCTGAGTGACCTG
CTGGGTCATCCCTTCTTTTGGACTTGGGAGAGCCGCTATAGGACGCTTCGGAATGTGGGA
AATGAATCCGACATCAAAACACGAAAATCTGAAAGTGAGATCCTCAGACTACTGCAACCT
GGGCCTTCTGAACATTCCAAAAGTTTTGACAAGTGGACGACTAAGATTAATGAATGTGTT
ATGAAAAAAATGAATAAGTTTTATGAAAAAAGAGGCAATTTCTACCAGAACACTGTGGGT
GATCTGCTAAAGTTCATCCGGAATTTGGGAGAACACATTGATGAAGAAAAGCATAAAAAG
ATGAAATTAAAAATTGGAGACCCTTCCCTGTATTTTCAGAAGACATTTCCAGATCTGGTG
ATCTATGTCTACACAAAACTACAGAACACAGAATATAGAAAGCATTTCCCCCAAACCCAC
AGTCCAAACAAACCTCAGTGTGATGGAGCTGGTGGGGCCAGTGGGTTGGCCAGCCCTGGG
TGCTGA
Protein Properties
Number of Residues 741
Molecular Weight 83532.1
Theoretical pI 6.64
Pfam Domain Function
Signals
  • ["None"]
Transmembrane Regions
  • ["None"]
Protein Sequence
>2-5A-dependent ribonuclease
MESRDHNNPQEGPTSSSGRRAAVEDNHLLIKAVQNEDVDLVQQLLEGGANVNFQEEEGGW
TPLHNAVQMSREDIVELLLRHGADPVLRKKNGATPFILAAIAGSVKLLKLFLSKGADVNE
CDFYGFTAFMEAAVYGKVKALKFLYKRGANVNLRRKTKEDQERLRKGGATALMDAAEKGH
VEVLKILLDEMGADVNACDNMGRNALIHALLSSDDSDVEAITHLLLDHGADVNVRGERGK
TPLILAVEKKHLGLVQRLLEQEHIEINDTDSDGKTALLLAVELKLKKIAELLCKRGASTD
CGDLVMTARRNYDHSLVKVLLSHGAKEDFHPPAEDWKPQSSHWGAALKDLHRIYRPMIGK
LKFFIDEKYKIADTSEGGIYLGFYEKQEVAVKTFCEGSPRAQREVSCLQSSRENSHLVTF
YGSESHRGHLFVCVTLCEQTLEACLDVHRGEDVENEEDEFARNVLSSIFKAVQELHLSCG
YTHQDLQPQNILIDSKKAAHLADFDKSIKWAGDPQEVKRDLEDLGRLVLYVVKKGSISFE
DLKAQSNEEVVQLSPDEETKDLIHRLFHPGEHVRDCLSDLLGHPFFWTWESRYRTLRNVG
NESDIKTRKSESEILRLLQPGPSEHSKSFDKWTTKINECVMKKMNKFYEKRGNFYQNTVG
DLLKFIRNLGEHIDEEKHKKMKLKIGDPSLYFQKTFPDLVIYVYTKLQNTEYRKHFPQTH
SPNKPQCDGAGGASGLASPGC
GenBank ID Protein Not Available
UniProtKB/Swiss-Prot ID Q05823
UniProtKB/Swiss-Prot Entry Name RN5A_HUMAN
PDB IDs
GenBank Gene ID L10381
GeneCard ID RNASEL
GenAtlas ID RNASEL
HGNC ID HGNC:10050
References
General References Not Available