| Identification |
|---|
| HMDB Protein ID
| CDBP02500 |
| Secondary Accession Numbers
| Not Available |
| Name
| Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B |
| Description
| Not Available |
| Synonyms
|
- Oligosaccharyl transferase subunit STT3B
- STT3-B
- Source of immunodominant MHC-associated peptides homolog
|
| Gene Name
| STT3B |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in oligosaccharyl transferase activity |
| Specific Function
| Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient cotranslational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation. Mediates glycosylation of the disease variant AMYL-TTR 'Asp-38' of TTR at 'Asn-118', leading to its degradation.
|
| GO Classification
|
| Biological Process |
| post-translational protein modification |
| protein N-linked glycosylation via asparagine |
| glycoprotein catabolic process |
| co-translational protein modification |
| ER-associated protein catabolic process |
| response to unfolded protein |
| Cellular Component |
| oligosaccharyltransferase complex |
| integral to membrane |
| Component |
| membrane |
| cell part |
| Function |
| transferase activity |
| transferase activity, transferring hexosyl groups |
| transferase activity, transferring glycosyl groups |
| oligosaccharyl transferase activity |
| catalytic activity |
| Molecular Function |
| dolichyl-diphosphooligosaccharide-protein glycotransferase activity |
| Process |
| metabolic process |
| macromolecule metabolic process |
| macromolecule modification |
| protein modification process |
| protein amino acid glycosylation |
|
| Cellular Location
|
- Endoplasmic reticulum membrane
- Multi-pass membrane protein
|
| Pathways
|
| Name | SMPDB/Pathwhiz | KEGG | | protein glycosylation | Not Available | Not Available | | N-Glycan biosynthesis | Not Available |  | | Protein processing in endoplasmic reticulum | Not Available |  |
|
| Gene Properties |
|---|
| Chromosome Location
| 3 |
| Locus
| 3p23 |
| SNPs
| STT3B |
| Gene Sequence
|
>2481 bp
ATGGCGGAGCCCTCGGCCCCGGAGAGCAAGCACAAGTCGTCCCTCAACTCGTCCCCGTGG
AGTGGCCTCATGGCCCTGGGAAACAGCCGGCACGGCCACCACGGGCCCGGGGCCCAGTGC
GCGCACAAGGCGGCGGGCGGCGCGGCGCCGCCGAAGCCGGCCCCGGCGGGGCTGTCCGGG
GGGCTGTCGCAGCCGGCTGGGTGGCAGTCGCTTCTCTCCTTCACCATCCTCTTCCTGGCC
TGGCTTGCCGGCTTCAGCTCGCGCCTCTTCGCCGTCATCCGCTTCGAAAGCATCATCCAC
GAGTTCGACCCGTGGTTTAACTATAGATCAACACATCATCTTGCATCTCATGGGTTCTAT
GAATTTTTAAATTGGTTTGATGAAAGAGCATGGTATCCACTAGGAAGAATAGTAGGTGGT
ACTGTTTACCCAGGGTTGATGATAACCGCTGGCCTTATTCATTGGATTTTAAATACATTG
AACATAACTGTTCACATAAGAGACGTATGTGTGTTCCTTGCACCAACTTTTAGCGGCCTT
ACATCTATATCTACTTTCCTGCTTACAAGAGAACTTTGGAACCAAGGAGCAGGACTTTTA
GCTGCTTGTTTTATTGCTATTGTACCAGGCTACATATCTCGGTCAGTAGCTGGATCCTTT
GATAATGAAGGCATTGCTATTTTTGCACTTCAGTTCACATACTATTTATGGGTAAAATCT
GTAAAAACTGGGTCAGTTTTTTGGACAATGTGCTGCTGCTTATCCTATTTCTATATGGTC
TCTGCTTGGGGTGGTTATGTATTTATCATCAATCTTATTCCACTGCATGTATTTGTGTTG
TTACTGATGCAGAGATACAGCAAAAGAGTCTACATAGCATATAGCACTTTCTACATTGTG
GGTTTAATATTATCAATGCAGATACCTTTTGTGGGATTCCAGCCAATCAGAACAAGTGAA
CACATGGCAGCTGCAGGTGTCTTTGCATTGCTGCAAGCTTATGCTTTCTTGCAGTATCTG
AGAGACCGATTAACAAAACAAGAGTTCCAGACCCTTTTCTTTTTGGGTGTATCACTAGCT
GCAGGTGCTGTGTTCCTTAGTGTCATCTATTTGACTTATACAGGTTACATTGCACCATGG
AGTGGCAGGTTTTATTCATTGTGGGATACTGGGTATGCAAAAATACACATTCCAATTATT
GCATCAGTGTCTGAGCATCAACCTACGACTTGGGTGTCTTTCTTCTTTGATCTACATATT
CTTGTATGTACCTTCCCAGCAGGCCTTTGGTTCTGCATCAAAAATATCAACGATGAAAGA
GTATTTGTTGCTCTATATGCAATCAGTGCTGTCTACTTTGCTGGAGTGATGGTGCGACTG
ATGTTGACTTTGACTCCAGTCGTGTGTATGCTGTCTGCAATTGCCTTTTCAAATGTTTTT
GAGCACTATTTGGGGGATGACATGAAAAGGGAAAATCCACCTGTGGAGGACAGCAGTGAT
GAGGATGACAAAAGAAACCAAGGAAATTTGTATGATAAGGCAGGTAAAGTGAGGAAACAT
GCAACTGAACAGGAAAAAACTGAAGAGGGATTAGGCCCTAATATAAAAAGCATTGTCACC
ATGTTGATGCTGATGCTATTGATGATGTTTGCTGTCCACTGTACCTGGGTCACAAGCAAT
GCCTACTCTAGTCCAAGTGTAGTCCTGGCCTCATACAATCATGATGGCACCAGGAATATC
TTAGATGATTTTAGAGAAGCTTACTTTTGGCTAAGGCAAAATACAGATGAACATGCACGA
GTAATGTCTTGGTGGGATTATGGCTATCAGATAGCTGGAATGGCTAATAGAACTACGTTG
GTGGATAATAACACCTGGAATAACAGCCACATAGCACTGGTGGGAAAAGCTATGTCTTCT
AATGAAACAGCAGCCTATAAAATCATGAGGACTCTAGATGTAGATTATGTTTTGGTTATT
TTTGGAGGGGTTATTGGCTATTCTGGTGATGATATCAACAAATTTCTCTGGATGGTTAGG
ATAGCTGAAGGAGAACATCCCAAAGACATTCGGGAAAGTGACTATTTTACCCCACAGGGA
GAATTCCGTGTAGACAAAGCAGGATCCCCTACTTTGTTGAATTGCCTTATGTATAAAATG
TCATACTACAGATTTGGAGAAATGCAGCTGGATTTTCGTACACCCCCAGGTTTTGACCGA
ACACGTAATGCTGAGATTGGAAATAAGGACATTAAATTCAAACATTTGGAAGAAGCCTTT
ACATCAGAACACTGGCTTGTTAGGATATATAAAGTAAAAGCACCTGATAACAGGGAGACA
TTAGATCACAAACCTCGAGTCACCAACATTTTCCCAAAACAGAAGTATTTGTCAAAGAAG
ACTACCAAAAGGAAGCGTGGCTACATTAAAAATAAGCTGGTTTTTAAGAAAGGCAAGAAA
ATATCTAAGAAGACTGTTTAA
|
| Protein Properties |
|---|
| Number of Residues
| 826 |
| Molecular Weight
| 93673.495 |
| Theoretical pI
| 8.911 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQCAHKAAGGAAPPKPAPAGLSG
GLSQPAGWQSLLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFY
EFLNWFDERAWYPLGRIVGGTVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGL
TSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKS
VKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIV
GLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLA
AGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHI
LVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVF
EHYLGDDMKRENPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVT
MLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHAR
VMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVI
FGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKM
SYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRET
LDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFKKGKKISKKTV
|
| External Links |
|---|
| GenBank ID Protein
| 30578410 |
| UniProtKB/Swiss-Prot ID
| Q8TCJ2 |
| UniProtKB/Swiss-Prot Entry Name
| STT3B_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| NM_178862.1 |
| GeneCard ID
| STT3B |
| GenAtlas ID
| STT3B |
| HGNC ID
| HGNC:30611 |
| References |
|---|
| General References
| Not Available |