| Identification |
|---|
| HMDB Protein ID
| CDBP02210 |
| Secondary Accession Numbers
| Not Available |
| Name
| Cholesterol 25-hydroxylase |
| Description
| Not Available |
| Synonyms
|
- Cholesterol 25-monooxygenase
- h25OH
|
| Gene Name
| CH25H |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in iron ion binding |
| Specific Function
| Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.
|
| GO Classification
|
| Biological Process |
| bile acid biosynthetic process |
| cholesterol metabolic process |
| sterol biosynthetic process |
| fatty acid biosynthetic process |
| Cellular Component |
| endoplasmic reticulum membrane |
| cytosol |
| integral to membrane |
| Component |
| endoplasmic reticulum |
| organelle |
| membrane-bounded organelle |
| intracellular membrane-bounded organelle |
| Function |
| ion binding |
| cation binding |
| metal ion binding |
| binding |
| catalytic activity |
| transition metal ion binding |
| iron ion binding |
| oxidoreductase activity |
| Molecular Function |
| iron ion binding |
| cholesterol 25-hydroxylase activity |
| steroid hydroxylase activity |
| Process |
| monocarboxylic acid metabolic process |
| fatty acid metabolic process |
| fatty acid biosynthetic process |
| oxidation reduction |
| metabolic process |
| cellular metabolic process |
| organic acid metabolic process |
| oxoacid metabolic process |
| carboxylic acid metabolic process |
|
| Cellular Location
|
- Endoplasmic reticulum membrane
- Multi-pass membrane protein
|
| Pathways
|
| Name | SMPDB/Pathwhiz | KEGG | | Primary bile acid biosynthesis | Not Available |  | | Bile Acid Biosynthesis |    | | | Congenital Bile Acid Synthesis Defect Type II |    | Not Available | | Congenital Bile Acid Synthesis Defect Type III |    | Not Available | | Familial Hypercholanemia (FHCA) |    | Not Available |
|
| Gene Properties |
|---|
| Chromosome Location
| 10 |
| Locus
| 10q23 |
| SNPs
| CH25H |
| Gene Sequence
|
>819 bp
ATGAGCTGCCACAACTGCTCCGACCCCCAGGTCCTTTGCAGCTCCGGGCAGCTGTTCCTG
CAGCCCCTCTGGGACCACCTGAGGAGCTGGGAGGCCCTCCTACAGTCGCCCTTCTTCCCG
GTCATCTTCTCCATCACCACATACGTGGGCTTTTGCCTGCCCTTCGTGGTCCTGGATATC
CTGTGCTCCTGGGTGCCCGCCCTGCGGCGCTACAAGATCCACCCTGACTTCTCGCCATCC
GCGCAGCAGCTGCTACCTTGCCTGGGGCAGACCCTCTACCAGCATGTGATGTTTGTGTTC
CCCGTGACGCTGCTGCATTGGGCCCGCAGCCCGGCCCTCCTGCCCCACGAAGCTCCCGAG
CTGCTCCTGCTGCTGCACCACATCCTGTTCTGCCTGCTACTCTTCGACATGGAGTTCTTC
GTGTGGCACCTGCTGCACCACAAGGTGCCCTGGCTGTACCGCACCTTCCACAAGGTGCAC
CACCAGAACTCGTCCTCGTTCGCGCTGGCAACGCAGTATATGAGCGTCTGGGAACTGTTT
TCTTTGGGCTTCTTCGACATGATGAACGTCACACTGCTCGGGTGCCACCCGCTCACCACC
CTGACCTTCCACGTGGTCAACATCTGGCTTTCCGTGGAGGACCACTCCGGCTACAACTTC
CCTTGGTCCACTCACAGACTGGTGCCCTTCGGGTGGTACGGGGGTGTGGTGCACCACGAC
CTGCATCACTCTCACTTTAACTGCAACTTCGCTCCGTACTTTACACACTGGGACAAAATA
CTGGGAACGCTGCGGACTGCATCTGTCCCAGCGCGGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 272 |
| Molecular Weight
| 31744.755 |
| Theoretical pI
| 7.249 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Cholesterol 25-hydroxylase
MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDI
LCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPE
LLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELF
SLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHD
LHHSHFNCNFAPYFTHWDKILGTLRTASVPAR
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| O95992 |
| UniProtKB/Swiss-Prot Entry Name
| CH25H_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| AF059214 |
| GeneCard ID
| CH25H |
| GenAtlas ID
| CH25H |
| HGNC ID
| HGNC:1907 |
| References |
|---|
| General References
| Not Available |