Cannabis Compound Database: Showing Protein Adenylyltransferase and sulfurtransferase MOCS3 (CDBP01735)

Identification Biological properties Gene properties Protein properties External links References XML Show 4 compounds

Identification

HMDB Protein ID

CDBP01735

Secondary Accession Numbers

Not Available

Name

Adenylyltransferase and sulfurtransferase MOCS3

Description

Not Available

Synonyms

Adenylyltransferase MOCS3
MPT synthase sulfurylase
Molybdenum cofactor synthesis protein 3
Molybdopterin synthase sulfurylase
Sulfur carrier protein MOCS2A adenylyltransferase
Sulfur carrier protein MOCS2A sulfurtransferase
Sulfurtransferase MOCS3

Gene Name

MOCS3

Protein Type

Enzyme

Biological Properties

General Function

Involved in catalytic activity

Specific Function

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

GO Classification

Biological Process
enzyme active site formation via L-cysteine persulfide
tRNA thio-modification
tRNA wobble uridine modification
water-soluble vitamin metabolic process
Mo-molybdopterin cofactor biosynthetic process
Cellular Component
cytosol
Function
binding
catalytic activity
Molecular Function
thiosulfate sulfurtransferase activity
nucleotidyltransferase activity
URM1 activating enzyme activity
metal ion binding
ATP binding
Process
metabolic process

Cellular Location

Cytoplasm
cytosol

Pathways

Name	SMPDB/Pathwhiz	KEGG
molybdopterin biosynthesis	Not Available	Not Available
5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis	Not Available	Not Available
Sulfur relay system	Not Available

Gene Properties

Chromosome Location

Locus

20q13.13

SNPs

MOCS3

Gene Sequence

>1383 bp
ATGGCTTCCCGGGAGGAGGTACTCGCCTTACAAGCTGAAGTTGCCCAACGTGAGGAGGAA
TTGAATTCGCTGAAGCAGAAGCTGGCGTCGGCTCTTTTGGCTGAGCAGGAACCGCAGCCA
GAACGGCTGGTTCCGGTGTCGCCGCTGCCGCCGAAGGCCGCTCTGTCCCGAGATGAGATT
CTGCGCTATAGCCGGCAGCTAGTGCTGCCCGAGCTGGGCGTGCACGGACAGCTGCGCCTG
GGGACCGCGTGCGTGCTAATCGTGGGCTGCGGTGGGCTCGGCTGTCCACTAGCGCAGTAC
TTGGCAGCGGCCGGCGTGGGCCGCCTTGGCCTTGTGGACTATGACGTGGTAGAGATGAGC
AACCTGGCCCGCCAAGTGCTGCATGGCGAGGCACTGGCTGGCCAGGCCAAGGCCTTTTCG
GCCGCCGCCTCGCTGCGCCGCCTCAATTCGGCAGTGGAATGCGTGCCGTACACTCAGGCC
CTTACGCCAGCCACTGCCCTAGACCTGGTCCGCCGATATGATGTGGTGGCTGACTGCTCG
GACAACGTGCCCACTCGCTACCTGGTTAATGACGCATGTGTGCTGGCGGGTCGGCCCCTC
GTGTCTGCCAGTGCCTTGCGCTTCGAGGGCCAAATCACAGTCTACCATTATGACGGTGGC
CCTTGCTATCGCTGCATATTCCCCCAACCACCCCCAGCGGAGACAGTGACCAACTGCGCG
GACGGCGGGGTGCTCGGTGTCGTTACCGGGGTCCTGGGCTGCCTGCAGGCCTTGGAAGTG
CTGAAAATCGCTGCGGGTCTGGGCCCCTCTTACAGTGGCAGCTTGTTGCTCTTTGATGCC
CTGAGAGGGCATTTCCGCTCTATTCGGCTGCGGAGCCGCAGGCTCGACTGTGCAGCTTGC
GGGGAACGGCCCACTGTGACTGATCTGCTGGACTATGAAGCCTTCTGTGGCTCCTCAGCC
ACTGATAAATGCCGCTCCCTGCAACTACTGAGCCCAGAGGAGCGTGTTTCTGTCACCGAC
TATAAGCGACTGCTGGATTCTGGGGCATTCCACCTGTTGCTGGACGTCAGGCCTCAGGTG
GAGGTGGACATTTGTCGTTTGCCTCATGCCCTACACATCCCTCTGAAACATTTGGAACGC
AGGGATGCGGAGAGCCTGAAACTCTTAAAAGAAGCAATCTGGGAAGAGAAGCAGGGCACA
CAAGAAGGGGCTGCTGTCCCCATTTATGTGATTTGCAAACTGGGAAATGACTCACAGAAA
GCCGTGAAGATCCTCCAGTCCTTATCAGCAGCTCAAGAGTTAGACCCTTTAACAGTTCGG
GATGTTGTGGGGGGCCTCATGGCCTGGGCTGCCAAAATCGATGGAACATTTCCACAGTAC
TGA

Protein Properties

Number of Residues

460

Molecular Weight

49668.685

Theoretical pI

6.209

Pfam Domain Function

ThiF (PF00899 )
Rhodanese (PF00581 )
MoeZ_MoeB (PF05237 )

Signals

Not Available

Transmembrane Regions

Not Available

Protein Sequence

>Adenylyltransferase and sulfurtransferase MOCS3
MASREEVLALQAEVAQREEELNSLKQKLASALLAEQEPQPERLVPVSPLPPKAALSRDEI
LRYSRQLVLPELGVHGQLRLGTACVLIVGCGGLGCPLAQYLAAAGVGRLGLVDYDVVEMS
NLARQVLHGEALAGQAKAFSAAASLRRLNSAVECVPYTQALTPATALDLVRRYDVVADCS
DNVPTRYLVNDACVLAGRPLVSASALRFEGQITVYHYDGGPCYRCIFPQPPPAETVTNCA
DGGVLGVVTGVLGCLQALEVLKIAAGLGPSYSGSLLLFDALRGHFRSIRLRSRRLDCAAC
GERPTVTDLLDYEAFCGSSATDKCRSLQLLSPEERVSVTDYKRLLDSGAFHLLLDVRPQV
EVDICRLPHALHIPLKHLERRDAESLKLLKEAIWEEKQGTQEGAAVPIYVICKLGNDSQK
AVKILQSLSAAQELDPLTVRDVVGGLMAWAAKIDGTFPQY

External Links

GenBank ID Protein

3851719

UniProtKB/Swiss-Prot ID

O95396

UniProtKB/Swiss-Prot Entry Name

MOCS3_HUMAN

PDB IDs