| Identification |
|---|
| HMDB Protein ID
| CDBP01204 |
| Secondary Accession Numbers
| Not Available |
| Name
| Bifunctional polynucleotide phosphatase/kinase |
| Description
| Not Available |
| Synonyms
|
- 2'(3')-polynucleotidase
- DNA 5'-kinase/3'-phosphatase
- Polynucleotide 3'-phosphatase
- Polynucleotide 5'-hydroxyl-kinase
- Polynucleotide kinase-3'-phosphatase
|
| Gene Name
| PNKP |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in ATP binding |
| Specific Function
| Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.
|
| GO Classification
|
| Biological Process |
| response to oxidative stress |
| nucleotide-excision repair, DNA damage removal |
| DNA-dependent DNA replication |
| response to radiation |
| DNA damage response, detection of DNA damage |
| Cellular Component |
| nucleolus |
| Molecular Function |
| endonuclease activity |
| ATP binding |
| double-stranded DNA binding |
| ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity |
| polynucleotide 3'-phosphatase activity |
| damaged DNA binding |
| nucleotide kinase activity |
|
| Cellular Location
|
- Nucleus
|
| Pathways
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 19 |
| Locus
| 19q13.3-q13.4 |
| SNPs
| PNKP |
| Gene Sequence
|
>1566 bp
ATGGGCGAGGTGGAGGCCCCGGGCCGCTTGTGGCTCGAGAGCCCCCCTGGGGGAGCGCCC
CCCATCTTCCTGCCCTCGGACGGGCAAGCCCTGGTCCTGGGCAGGGGACCCCTGACCCAG
GTTACGGACCGGAAGTGCTCCAGAACTCAAGTGGAGCTGGTCGCAGATCCTGAGACCCGG
ACAGTGGCAGTGAAACAGCTGGGAGTTAACCCCTCAACTACCGGGACCCAGGAGTTGAAG
CCGGGGTTGGAGGGCTCTCTGGGGGTGGGGGACACACTGTATTTGGTCAATGGCCTCCAC
CCACTGACCCTGCGCTGGGAAGAGACCCGCACACCAGAATCCCAGCCAGATACTCCGCCT
GGCACCCCTCTGGTGTCCCAAGATGAGAAGAGAGATGCTGAGCTGCCGAAGAAGCGTATG
CGGAAGTCAAACCCCGGCTGGGAGAACTTGGAGAAGTTGCTAGTGTTCACCGCAGCTGGG
GTGAAACCCCAGGGCAAGGTGGCTGGCTTTGATCTGGACGGGACGCTCATCACCACACGC
TCTGGGAAGGTCTTTCCCACTGGCCCCAGTGACTGGAGGATCTTGTACCCAGAGATTCCC
CGTAAGCTCCGAGAGCTGGAAGCCGAGGGCTACAAGCTGGTGATCTTCACCAACCAGATG
AGCATCGGGCGCGGGAAGCTGCCAGCCGAGGAGTTCAAGGCCAAGGTGGAGGCTGTGGTG
GAGAAGCTGGGGGTCCCCTTCCAGGTGCTGGTGGCCACGCACGCAGGCTTGTACCGGAAG
CCGGTGACGGGCATGTGGGACCATCTGCAGGAGCAGGCCAACGACGGCACGCCCATATCC
ATCGGGGACAGCATCTTTGTGGGAGACGCAGCCGGACGCCCGGCCAACTGGGCCCCGGGG
CGGAAGAAGAAAGACTTCTCCTGCGCCGATCGCCTGTTTGCCCTCAACCTTGGCCTGCCC
TTCGCCACGCCTGAGGAGTTCTTTCTCAAGTGGCCAGCAGCCGGCTTCGAGCTCCCAGCC
TTTGATCCGAGGACTGTCTCCCGCTCAGGGCCTCTCTGCCTCCCCGAGTCCAGGGCCCTC
CTGAGCGCCAGCCCGGAGGTGGTTGTCGCAGTGGGATTCCCTGGGGCCGGGAAGTCCACC
TTTCTCAAGAAGCACCTCGTGTCGGCCGGATATGTCCACGTGAACAGGGACACGCTAGGC
TCCTGGCAGCGCTGTGTGACCACGTGTGAGACAGCCCTGAAGCAAGGGAAACGGGTCGCC
ATCGACAACACAAACCCAGACGCCGCGAGCCGCGCCAGGTACGTCCAGTGTGCCCGAGCC
GCGGGCGTCCCCTGCCGCTGCTTCCTCTTCACCGCCACTCTGGAGCAGGCGCGCCACAAC
AACCGGTTTCGAGAGATGACGGACTCCTCTCATATCCCCGTGTCAGACATGGTCATGTAT
GGCTACAGGAAGCAGTTCGAGGCCCCAACGCTGGCTGAAGGCTTCTCTGCCATCCTGGAG
ATCCCGTTCCGGCTATGGGTGGAGCCGAGGCTGGGGCGGCTGTACTGCCAGTTCTCCGAG
GGCTGA
|
| Protein Properties |
|---|
| Number of Residues
| 521 |
| Molecular Weight
| 57075.9 |
| Theoretical pI
| 8.46 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Bifunctional polynucleotide phosphatase/kinase
MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETR
TVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPP
GTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTR
SGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVV
EKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPG
RKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRAL
LSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVA
IDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMY
GYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG
|
| External Links |
|---|
| GenBank ID Protein
| 31543419 |
| UniProtKB/Swiss-Prot ID
| Q96T60 |
| UniProtKB/Swiss-Prot Entry Name
| PNKP_HUMAN |
| PDB IDs
|
|
| GenBank Gene ID
| NM_007254.3 |
| GeneCard ID
| PNKP |
| GenAtlas ID
| PNKP |
| HGNC ID
| HGNC:9154 |
| References |
|---|
| General References
| Not Available |