| Identification |
|---|
| HMDB Protein ID
| CDBP01110 |
| Secondary Accession Numbers
| Not Available |
| Name
| Mitotic checkpoint serine/threonine-protein kinase BUB1 |
| Description
| Not Available |
| Synonyms
|
- BUB1A
- hBUB1
|
| Gene Name
| BUB1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in protein kinase activity |
| Specific Function
| Serine/threonine-protein kinase that performs 2 crucial functions during mitosis:it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis |
| GO Classification
|
| Function |
| protein serine/threonine kinase activity |
| binding |
| catalytic activity |
| transferase activity |
| transferase activity, transferring phosphorus-containing groups |
| kinase activity |
| nucleoside binding |
| purine nucleoside binding |
| adenyl nucleotide binding |
| adenyl ribonucleotide binding |
| atp binding |
| protein kinase activity |
| Process |
| phosphorus metabolic process |
| phosphate metabolic process |
| metabolic process |
| cellular metabolic process |
| protein amino acid phosphorylation |
| phosphorylation |
|
| Cellular Location
|
- Nucleus
- Chromosome
- centromere
- kinetochore
|
| Pathways
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Chromosome:2 |
| Locus
| 2q14 |
| SNPs
| BUB1 |
| Gene Sequence
|
>3258 bp
ATGGACACCCCGGAAAATGTCCTTCAGATGCTTGAAGCCCACATGCAGAGCTACAAGGGC
AATGACCCTCTTGGTGAATGGGAAAGATACATACAGTGGGTAGAAGAGAATTTTCCTGAG
AATAAAGAATACTTGATAACTTTACTAGAACATTTAATGAAGGAATTTTTAGATAAGAAG
AAATACCACAATGACCCAAGATTCATCAGTTATTGTTTAAAATTTGCTGAGTACAACAGT
GACCTCCATCAATTTTTTGAGTTTCTGTACAACCATGGGATTGGAACCCTGTCATCCCCT
CTGTACATTGCCTGGGCGGGGCATCTGGAAGCCCAAGGAGAGCTGCAGCATGCCAGTGCT
GTCCTTCAGAGAGGAATTCAAAACCAGGCTGAACCCAGAGAGTTCCTGCAACAACAATAC
AGGTTATTTCAGACACGCCTCACTGAAACCCATTTGCCAGCTCAAGCTAGAACCTCAGAA
CCTCTGCATAATGTTCAGGTTTTAAATCAAATGATAACATCAAAATCAAATCCAGGAAAT
AACATGGCCTGCATTTCTAAGAATCAGGGTTCAGAGCTTTCTGGAGTGATATCTTCAGCT
TGTGATAAAGAGTCAAATATGGAACGAAGAGTGATCACGATTTCTAAATCAGAATATTCT
GTGCACTCATCTTTGGCATCCAAAGTTGATGTTGAGCAGGTTGTTATGTATTGCAAGGAG
AAGCTTATTCGTGGGGAATCAGAATTTTCCTTTGAAGAATTGAGAGCCCAGAAATACAAT
CAACGGAGAAAGCATGAGCAATGGGTAAATGAAGACAGACATTATATGAAAAGGAAAGAA
GCAAATGCTTTTGAAGAACAGCTATTAAAACAGAAAATGGATGAACTTCATAAGAAGTTG
CATCAGGTGGTGGAGACATCCCATGAGGATCTGCCCGCTTCCCAGGAAAGGTCCGAGGTT
AATCCAGCACGTATGGGGCCAAGTGTAGGCTCCCAGCAGGAACTGAGAGCGCCATGTCTT
CCAGTAACCTATCAGCAGACACCAGTGAACATGGAAAAGAACCCAAGAGAGGCACCTCCT
GTTGTTCCTCCTTTGGCAAATGCTATTTCTGCAGCTTTGGTGTCCCCAGCCACCAGCCAG
AGCATTGCTCCTCCTGTTCCTTTGAAAGCCCAGACAGTAACAGACTCCATGTTTGCAGTG
GCCAGCAAAGATGCTGGATGTGTGAATAAGAGTACTCATGAATTCAAGCCACAGAGTGGA
GCAGAGATCAAAGAAGGGTGTGAAACACATAAGGTTGCCAACACAAGTTCTTTTCACACA
ACTCCAAACACATCACTGGGAATGGTTCAGGCAACGCCATCCAAAGTGCAGCCATCACCC
ACCGTGCACACAAAAGAAGCATTAGGTTTCATCATGAATATGTTTCAGGCTCCTACACTT
CCTGATATTTCTGATGACAAAGATGAATGGCAATCTCTAGATCAAAATGAAGATGCATTT
GAAGCCCAGTTTCAAAAAAATGTAAGGTCATCTGGGGCTTGGGGAGTCAATAAGATCATC
TCTTCTTTGTCATCTGCTTTTCATGTGTTTGAAGATGGAAACAAAGAAAATTATGGATTA
CCACAGCCTAAAAATAAACCCACAGGAGCCAGGACCTTTGGAGAACGCTCTGTCAGCAGA
CTTCCTTCAAAACCAAAGGAGGAAGTGCCTCATGCTGAAGAGTTTTTGGATGACTCAACT
GTATGGGGTATTCGCTGCAACAAAACCCTGGCACCCAGTCCTAAGAGCCCAGGAGACTTC
ACATCTGCTGCACAACTTGCGTCTACACCATTCCACAAGCTTCCAGTGGAGTCAGTGCAC
ATTTTAGAAGATAAAGAAAATGTGGTAGCAAAACAGTGTACCCAGGCGACTTTGGATTCT
TGTGAGGAAAACATGGTGGTGCCTTCAAGGGATGGAAAATTCAGTCCAATTCAAGAGAAA
AGCCCAAAACAGGCCTTGTCGTCTCACATGTATTCAGCATCCTTACTTCGTCTGAGCCAG
CCTGCTGCAGGTGGGGTACTTACCTGTGAGGCAGAGTTGGGCGTTGAGGCTTGCAGACTC
ACAGACACTGACGCTGCCATTGCAGAAGATCCACCAGATGCTATTGCTGGGCTCCAAGCA
GAATGGATGCAGATGAGTTCACTTGGGACTGTTGATGCTCCAAACTTCATTGTTGGGAAC
CCATGGGATGATAAGCTGATTTTCAAACTTTTATCTGGGCTTTCTAAACCAGTGAGTTCC
TATCCAAATACTTTTGAATGGCAATGTAAACTTCCAGCCATCAAGCCCAAGACTGAATTT
CAATTGGGTTCTAAGCTGGTCTATGTCCATCACCTTCTTGGAGAAGGAGCCTTTGCCCAG
GTGTACGAAGCTACCCAGGGAGATCTGAATGATGCTAAAAATAAACAGAAATTTGTTTTA
AAGGTCCAAAAGCCTGCCAACCCCTGGGAATTCTACATTGGGACCCAGTTGATGGAAAGA
CTAAAGCCATCTATGCAGCACATGTTTATGAAGTTCTATTCTGCCCACTTATTCCAGAAT
GGCAGTGTATTAGTAGGAGAGCTCTACAGCTATGGAACATTATTAAATGCCATTAACCTC
TATAAAAATACCCCTGAAAAAGTGATGCCTCAAGGTCTTGTCATCTCTTTTGCTATGAGA
ATGCTTTACATGATTGAGCAAGTGCATGACTGTGAAATCATTCATGGAGACATTAAACCA
GACAATTTCATACTTGGAAACGGATTTTTGGAACAGGATGATGAAGATGATTTATCTGCT
GGCTTGGCACTGATTGACCTGGGTCAGAGTATAGATATGAAACTTTTTCCAAAAGGAACT
ATATTCACAGCAAAGTGTGAAACATCTGGTTTTCAGTGTGTTGAGATGCTCAGCAACAAA
CCATGGAACTACCAGATCGATTACTTTGGGGTTGCTGCAACAGTATATTGCATGCTCTTT
GGCACTTACATGAAAGTGAAAAATGAAGGAGGAGAGTGTAAGCCTGAAGGTCTTTTTAGA
AGGCTTCCTCATTTGGATATGTGGAATGAATTTTTTCATGTTATGTTGAATATTCCAGAT
TGTCATCATCTTCCATCTTTGGATTTGTTAAGGCAAAAGCTGAAGAAAGTATTTCAACAA
CACTATACTAACAAGATTAGGGCCCTACGTAATAGGCTAATTGTACTGCTCTTAGAATGT
AAGCGTTCACGAAAATAA
|
| Protein Properties |
|---|
| Number of Residues
| 1085 |
| Molecular Weight
| 122374.4 |
| Theoretical pI
| 6.44 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>Mitotic checkpoint serine/threonine-protein kinase BUB1
MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKK
KYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASA
VLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGN
NMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVDVEQVVMYCKE
KLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKL
HQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVNMEKNPREAPP
VVPPLANAISAALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSG
AEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTL
PDISDDKDEWQSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSSAFHVFEDGNKENYGL
PQPKNKPTGARTFGERSVSRLPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPKSPGDF
TSAAQLASTPFHKLPVESVHILEDKENVVAKQCTQATLDSCEENMVVPSRDGKFSPIQEK
SPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRLTDTDAAIAEDPPDAIAGLQA
EWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEF
QLGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMER
LKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMR
MLYMIEQVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGT
IFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFR
RLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLEC
KRSRK
|
| External Links |
|---|
| GenBank ID Protein
| 4757878 |
| UniProtKB/Swiss-Prot ID
| O43683 |
| UniProtKB/Swiss-Prot Entry Name
| BUB1_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| NM_004336.3 |
| GeneCard ID
| BUB1 |
| GenAtlas ID
| BUB1 |
| HGNC ID
| HGNC:1148 |
| References |
|---|
| General References
| Not Available |