| Identification |
|---|
| HMDB Protein ID
| CDBP00960 |
| Secondary Accession Numbers
| Not Available |
| Name
| Hypoxia-inducible factor 1-alpha inhibitor |
| Description
| Not Available |
| Synonyms
|
- FIH-1
- Factor inhibiting HIF-1
- Hypoxia-inducible factor asparagine hydroxylase
|
| Gene Name
| HIF1AN |
| Protein Type
| Transcription Factor |
| Biological Properties |
|---|
| General Function
| Involved in metal ion binding |
| Specific Function
| Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
|
| GO Classification
|
| Biological Process |
| positive regulation of myoblast differentiation |
| positive regulation of vasculogenesis |
| transcription, DNA-dependent |
| peptidyl-aspartic acid hydroxylation |
| negative regulation of Notch signaling pathway |
| negative regulation of transcription from RNA polymerase II promoter in response to hypoxia |
| peptidyl-asparagine hydroxylation |
| peptidyl-histidine hydroxylation |
| Cellular Component |
| cytosol |
| perinuclear region of cytoplasm |
| nucleus |
| Molecular Function |
| oxygen sensor activity |
| oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen |
| cofactor binding |
| iron ion binding |
| carboxylic acid binding |
| zinc ion binding |
| protein homodimerization activity |
| peptidyl-asparagine 3-dioxygenase activity |
| peptidyl-histidine dioxygenase activity |
|
| Cellular Location
|
- Nucleus (Potential)
|
| Pathways
|
| Name | SMPDB/Pathwhiz | KEGG | | Glutaminolysis and Cancer |    | Not Available |
|
| Gene Properties |
|---|
| Chromosome Location
| 10 |
| Locus
| 10q24 |
| SNPs
| HIF1AN |
| Gene Sequence
|
>1050 bp
ATGGCGGCGACAGCGGCGGAGGCTGTGGCCTCTGGCTCTGGAGAGCCCCGGGAGGAGGCT
GGAGCCCTCGGCCCCGCCTGGGATGAATCCCAGTTGCGCAGTTATAGCTTCCCGACTAGG
CCCATTCCGCGTCTGAGTCAGAGCGACCCCCGGGCAGAGGAGCTTATTGAGAATGAGGAG
CCTGTGGTGCTGACCGACACAAATCTTGTGTATCCTGCCCTGAAATGGGACCTTGAATAC
CTGCAAGAGAATATTGGCAATGGAGACTTCTCTGTGTACAGTGCCAGCACCCACAAGTTC
TTGTACTATGATGAGAAGAAGATGGCCAATTTCCAGAACTTTAAGCCGAGGTCCAACAGG
GAAGAAATGAAATTTCATGAGTTCGTTGAGAAACTGCAGGATATACAGCAGCGAGGAGGG
GAAGAGAGGTTGTATCTGCAGCAAACGCTCAATGACACTGTGGGCAGGAAGATTGTCATG
GACTTCTTAGGTTTTAACTGGAACTGGATTAATAAGCAACAGGGAAAGCGTGGCTGGGGG
CAGCTTACCTCTAACCTGCTGCTCATTGGCATGGAAGGAAATGTGACACCTGCTCACTAT
GATGAGCAGCAGAACTTTTTTGCTCAGATAAAAGGTTACAAACGATGCATCTTATTCCCT
CCGGATCAGTTCGAGTGCCTCTACCCATACCCTGTTCATCACCCATGTGACAGACAGAGC
CAGGTGGACTTTGACAATCCCGACTACGAGAGGTTCCCTAATTTCCAAAATGTGGTTGGT
TACGAAACAGTGGTTGGCCCTGGTGATGTTCTTTACATCCCAATGTACTGGTGGCATCAC
ATAGAGTCATTACTAAATGGGGGGATTACCATCACTGTGAACTTCTGGTATAAGGGGGCT
CCCACCCCTAAGAGAATTGAATATCCTCTCAAAGCTCATCAGAAAGTGGCCATAATGAGA
AACATTGAGAAGATGCTTGGAGAGGCCTTGGGGAACCCACAAGAGGTGGGGCCCTTGTTG
AACACAATGATCAAGGGCCGATACAACTAG
|
| Protein Properties |
|---|
| Number of Residues
| 349 |
| Molecular Weight
| 40285.25 |
| Theoretical pI
| 5.57 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Hypoxia-inducible factor 1-alpha inhibitor
MAATAAEAVASGSGEPREEAGALGPAWDESQLRSYSFPTRPIPRLSQSDPRAEELIENEE
PVVLTDTNLVYPALKWDLEYLQENIGNGDFSVYSASTHKFLYYDEKKMANFQNFKPRSNR
EEMKFHEFVEKLQDIQQRGGEERLYLQQTLNDTVGRKIVMDFLGFNWNWINKQQGKRGWG
QLTSNLLLIGMEGNVTPAHYDEQQNFFAQIKGYKRCILFPPDQFECLYPYPVHHPCDRQS
QVDFDNPDYERFPNFQNVVGYETVVGPGDVLYIPMYWWHHIESLLNGGITITVNFWYKGA
PTPKRIEYPLKAHQKVAIMRNIEKMLGEALGNPQEVGPLLNTMIKGRYN
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| Q9NWT6 |
| UniProtKB/Swiss-Prot Entry Name
| HIF1N_HUMAN |
| PDB IDs
|
|
| GenBank Gene ID
| AF395830 |
| GeneCard ID
| HIF1AN |
| GenAtlas ID
| HIF1AN |
| HGNC ID
| HGNC:17113 |
| References |
|---|
| General References
| Not Available |