Identification |
HMDB Protein ID
| CDBP00557 |
Secondary Accession Numbers
| Not Available |
Name
| DNA ligase 4 |
Description
| Not Available |
Synonyms
|
- DNA ligase IV
- Polydeoxyribonucleotide synthase [ATP] 4
|
Gene Name
| LIG4 |
Protein Type
| Enzyme |
Biological Properties |
General Function
| Involved in DNA ligase (ATP) activity |
Specific Function
| Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
|
GO Classification
|
Biological Process |
DNA ligation involved in DNA recombination |
response to X-ray |
double-strand break repair via nonhomologous end joining |
viral reproduction |
immunoglobulin V(D)J recombination |
central nervous system development |
negative regulation of neuron apoptotic process |
neuron apoptotic process |
positive regulation of fibroblast proliferation |
positive regulation of neurogenesis |
pro-B cell differentiation |
single strand break repair |
cell cycle |
somatic stem cell maintenance |
cell division |
T cell differentiation in thymus |
DNA ligation involved in DNA repair |
T cell receptor V(D)J recombination |
lagging strand elongation |
response to gamma radiation |
nucleotide-excision repair, DNA gap filling |
cell proliferation |
cellular response to lithium ion |
in utero embryonic development |
chromosome organization |
isotype switching |
Cellular Component |
condensed chromosome |
DNA ligase IV complex |
DNA-dependent protein kinase-DNA ligase 4 complex |
focal adhesion |
cytoplasm |
plasma membrane |
nucleoplasm |
Component |
cell part |
intracellular |
Function |
adenyl nucleotide binding |
adenyl ribonucleotide binding |
atp binding |
ligase activity |
dna ligase (atp) activity |
ligase activity, forming phosphoric ester bonds |
dna ligase activity |
nucleic acid binding |
dna binding |
binding |
catalytic activity |
nucleoside binding |
purine nucleoside binding |
Molecular Function |
DNA ligase (ATP) activity |
metal ion binding |
ATP binding |
DNA binding |
Process |
dna metabolic process |
dna replication |
dna ligation |
dna ligation involved in dna repair |
dna recombination |
dna repair |
metabolic process |
macromolecule metabolic process |
cellular macromolecule metabolic process |
|
Cellular Location
|
- Nucleus
|
Pathways
|
Name | SMPDB/Pathwhiz | KEGG | Non-homologous end-joining | Not Available | ![Map03450](https://www.genome.jp/kegg/misc/thumbnail/map03450.gif) |
|
Gene Properties |
Chromosome Location
| 13 |
Locus
| 13q33-q34 |
SNPs
| LIG4 |
Gene Sequence
|
>2736 bp
ATGGCTGCCTCACAAACTTCACAAACTGTTGCATCTCACGTTCCTTTTGCAGATTTGTGT
TCAACTTTAGAACGAATACAGAAAAGTAAAGGACGTGCAGAAAAAATCAGACACTTCAGG
GAATTTTTAGATTCTTGGAGAAAATTTCATGATGCTCTTCATAAGAACCACAAAGATGTC
ACAGACTCTTTTTATCCAGCAATGAGACTAATTCTTCCTCAGCTAGAAAGAGAGAGAATG
GCCTATGGAATTAAAGAAACTATGCTTGCTAAGCTTTATATTGAGTTGCTTAATTTACCT
AGAGATGGAAAAGATGCCCTCAAACTTTTAAACTACAGAACACCCACTGGAACTCATGGA
GATGCTGGAGACTTTGCAATGATTGCATATTTTGTGTTGAAGCCAAGATGTTTACAGAAA
GGAAGTTTAACCATACAGCAAGTAAACGACCTTTTAGACTCAATTGCCAGCAATAATTCT
GCTAAAAGAAAAGACCTAATAAAAAAGAGCCTTCTTCAACTTATAACTCAGAGTTCAGCA
CTTGAGCAAAAGTGGCTTATACGGATGATCATAAAGGATTTAAAGCTTGGTGTTAGTCAG
CAAACTATCTTTTCTGTTTTTCATAATGATGCTGCTGAGTTGCATAATGTCACTACAGAT
CTGGAAAAAGTCTGTAGGCAACTGCATGATCCTTCTGTAGGACTCAGTGATATTTCTATC
ACTTTATTTTCTGCATTTAAACCAATGCTAGCTGCTATTGCAGATATTGAGCACATTGAG
AAGGATATGAAACATCAGAGTTTCTACATAGAAACCAAGCTAGATGGTGAACGTATGCAA
ATGCACAAAGATGGAGATGTATATAAATACTTCTCTCGAAATGGATATAACTACACTGAT
CAGTTTGGTGCTTCTCCTACTGAAGGTTCTCTTACCCCATTCATTCATAATGCATTCAAA
GCAGATATACAAATCTGTATTCTTGATGGTGAGATGATGGCCTATAATCCTAATACACAA
ACTTTCATGCAAAAGGGAACTAAGTTTGATATTAAAAGAATGGTAGAGGATTCTGATCTG
CAAACTTGTTATTGTGTTTTTGATGTATTGATGGTTAATAATAAAAAGCTAGGGCATGAG
ACTCTGAGAAAGAGGTATGAGATTCTTAGTAGTATTTTTACACCAATTCCAGGTAGAATA
GAAATAGTGCAGAAAACACAAGCTCATACTAAGAATGAAGTAATTGATGCATTGAATGAA
GCAATAGATAAAAGAGAAGAGGGAATTATGGTAAAACAACCTCTATCCATCTACAAGCCA
GACAAAAGAGGTGAAGGGTGGTTAAAAATTAAACCAGAGTATGTCAGTGGACTAATGGAT
GAATTGGACATTTTAATTGTTGGAGGATATTGGGGTAAAGGATCACGGGGTGGAATGATG
TCTCATTTTCTGTGTGCAGTAGCAGAGAAGCCCCCTCCTGGTGAGAAGCCATCTGTGTTT
CATACTCTCTCTCGTGTTGGGTCTGGCTGCACCATGAAAGAACTGTATGATCTGGGTTTG
AAATTGGCCAAGTATTGGAAGCCTTTTCATAGAAAAGCTCCACCAAGCAGCATTTTATGT
GGAACAGAGAAGCCAGAAGTATACATTGAACCTTGTAATTCTGTCATTGTTCAGATTAAA
GCAGCAGAGATCGTACCCAGTGATATGTATAAAACTGGCTGCACCTTGCGTTTTCCACGA
ATTGAAAAGATAAGAGATGACAAGGAGTGGCATGAGTGCATGACCCTGGACGACCTAGAA
CAACTTAGGGGGAAGGCATCTGGTAAGCTCGCATCTAAACACCTTTATATAGGTGGTGAT
GATGAACCACAAGAAAAAAAGCGGAAAGCTGCCCCAAAGATGAAGAAAGTTATTGGAATT
ATTGAGCACTTAAAAGCACCTAACCTTACTAACGTTAACAAAATTTCTAATATATTTGAA
GATGTAGAGTTTTGTGTTATGAGTGGAACAGATAGCCAGCCAAAGCCTGACCTGGAGAAC
AGAATTGCAGAATTTGGTGGTTATATAGTACAAAATCCAGGCCCAGACACGTACTGTGTA
ATTGCAGGGTCTGAGAACATCAGAGTGAAAAACATAATTTTGTCAAATAAACATGATGTT
GTCAAGCCTGCATGGCTTTTAGAATGTTTTAAGACCAAAAGCTTTGTACCATGGCAGCCT
CGCTTTATGATTCATATGTGCCCATCAACCAAAGAACATTTTGCCCGTGAATATGATTGC
TATGGTGATAGTTATTTCATTGATACAGACTTGAACCAACTGAAGGAAGTATTCTCAGGA
ATTAAAAATTCTAACGAGCAGACTCCTGAAGAAATGGCTTCTCTGATTGCTGATTTAGAA
TATCGGTATTCCTGGGATTGCTCTCCTCTCAGTATGTTTCGACGCCACACCGTTTATTTG
GACTCGTATGCTGTTATTAATGACCTGAGTACCAAAAATGAGGGGACAAGGTTAGCTATT
AAAGCCTTGGAGCTTCGGTTTCATGGAGCAAAAGTAGTTTCTTGTTTAGCTGAGGGAGTG
TCTCATGTAATAATTGGGGAAGATCATAGTCGTGTTGCAGATTTTAAAGCTTTTAGAAGA
ACTTTTAAGAGAAAGTTTAAAATCCTAAAAGAAAGTTGGGTAACTGATTCAATAGACAAG
TGTGAATTACAAGAAGAAAACCAGTATTTGATTTAA
|
Protein Properties |
Number of Residues
| 911 |
Molecular Weight
| 103969.85 |
Theoretical pI
| 7.959 |
Pfam Domain Function
|
|
Signals
|
Not Available
|
Transmembrane Regions
|
Not Available
|
Protein Sequence
|
>DNA ligase 4
MAASQTSQTVASHVPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDV
TDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHG
DAGDFAMIAYFVLKPRCLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSA
LEQKWLIRMIIKDLKLGVSQQTIFSVFHNDAAELHNVTTDLEKVCRQLHDPSVGLSDISI
TLFSAFKPMLAAIADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTD
QFGASPTEGSLTPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVEDSDL
QTCYCVFDVLMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNE
AIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMM
SHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILC
GTEKPEVYIEPCNSVIVQIKAAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLE
QLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFE
DVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDV
VKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSG
IKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAI
KALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDK
CELQEENQYLI
|
External Links |
GenBank ID Protein
| 148539894 |
UniProtKB/Swiss-Prot ID
| P49917 |
UniProtKB/Swiss-Prot Entry Name
| DNLI4_HUMAN |
PDB IDs
|
|
GenBank Gene ID
| NM_001098268.1 |
GeneCard ID
| LIG4 |
GenAtlas ID
| LIG4 |
HGNC ID
| HGNC:6601 |
References |
General References
| Not Available |