| Identification |
|---|
| HMDB Protein ID
| CDBP00511 |
| Secondary Accession Numbers
| Not Available |
| Name
| Cystathionine gamma-lyase |
| Description
| Not Available |
| Synonyms
|
- Gamma-cystathionase
- Cysteine-protein sulfhydrase
|
| Gene Name
| CTH |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in pyridoxal phosphate binding |
| Specific Function
| Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.
|
| GO Classification
|
| Biological Process |
| cysteine biosynthetic process |
| endoplasmic reticulum unfolded protein response |
| glutathione metabolic process |
| protein sulfhydration |
| protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine |
| positive regulation of I-kappaB kinase/NF-kappaB cascade |
| negative regulation of cell growth |
| protein homotetramerization |
| negative regulation of cell proliferation |
| positive regulation of NF-kappaB transcription factor activity |
| hydrogen sulfide biosynthetic process |
| transsulfuration |
| cellular nitrogen compound metabolic process |
| sulfur amino acid catabolic process |
| Cellular Component |
| cytosol |
| nucleus |
| Function |
| pyridoxal phosphate binding |
| binding |
| catalytic activity |
| cofactor binding |
| Molecular Function |
| cystathionine beta-lyase activity |
| cystathionine gamma-lyase activity |
| homocysteine desulfhydrase activity |
| L-cysteine desulfhydrase activity |
| L-cystine L-cysteine-lyase (deaminating) |
| pyridoxal phosphate binding |
| Process |
| metabolic process |
| cellular metabolic process |
| cellular amino acid and derivative metabolic process |
| cellular amino acid metabolic process |
|
| Cellular Location
|
- Cytoplasm
|
| Pathways
|
| Name | SMPDB/Pathwhiz | KEGG | | L-cysteine biosynthesis | Not Available | Not Available | | Glycine, serine and threonine metabolism | Not Available |  | | Cysteine and methionine metabolism | Not Available |  | | Selenocompound metabolism | Not Available |  | | Selenoamino Acid Metabolism |    | |
|
| Gene Properties |
|---|
| Chromosome Location
| 1 |
| Locus
| 1p31.1 |
| SNPs
| CTH |
| Gene Sequence
|
>1218 bp
ATGCAGGAAAAAGACGCCTCCTCACAAGGTTTCCTGCCACACTTCCAACATTTCGCCACG
CAGGCGATCCATGTGGGCCAGGATCCAGAGCAATGGACCTCCAGGGCTGTAGTGCCCCCC
ATCTCACTGTCCACCACGTTCAAGCAAGGGGCGCCTGGCCAGCACTCGGGTTTTGAATAT
AGCCGTTCTGGAAATCCCACTAGGAATTGCCTTGAAAAAGCAGTGGCAGCACTGGATGGG
GCTAAGTACTGTTTGGCCTTTGCTTCAGGTTTAGCAGCCACTGTAACTATTACCCATCTT
TTAAAAGCAGGAGACCAAATTATTTGTATGGATGATGTGTATGGAGGTACAAACAGGTAC
TTCAGGCAAGTGGCATCTGAATTTGGATTAAAGATTTCTTTTGTTGATTGTTCCAAAATC
AAATTACTAGAGGCAGCAATTACACCAGAAACCAAGCTTGTTTGGATCGAAACCCCCACA
AACCCCACCCAGAAGGTGATTGACATTGAAGGCTGTGCACATATTGTCCATAAGCATGGA
GACATTATTTTGGTCGTGGATAACACTTTTATGTCACCATATTTCCAGCGCCCTTTGGCT
CTGGGAGCTGATATTTCTATGTATTCTGCAACAAAATACATGAATGGCCACAGTGATGTT
GTAATGGGCCTGGTGTCTGTTAATTGTGAAAGCCTTCATAATAGACTTCGTTTCTTGCAA
AACTCTCTTGGAGCAGTTCCATCTCCTATTGATTGTTACCTCTGCAATCGAGGTCTGAAG
ACTCTACATGTCCGAATGGAAAAGCATTTCAAAAACGGAATGGCAGTTGCCCAGTTCCTG
GAATCTAATCCTTGGGTAGAAAAGGTTATTTATCCTGGGCTGCCCTCTCATCCACAGCAT
GAGTTGGTGAAGCGTCAGTGTACAGGTTGTACAGGGATGGTCACCTTTTATATTAAGGGC
ACTCTTCAGCATGCTGAGATTTTCCTCAAGAACCTAAAGCTATTTACTCTGGCCGAGAGC
TTGGGAGGATTCGAAAGCCTTGCTGAGCTTCCGGCAATCATGACTCATGCATCAGTTCTT
AAGAATGACAGAGATGTCCTTGGAATTAGTGACACACTGATTCGACTTTCTGTGGGCTTA
GAGGATGAGGAAGACCTACTGGAAGATCTAGATCAAGCTTTGAAGGCAGCACACCCTCCA
AGTGGAATTCACAGCTAG
|
| Protein Properties |
|---|
| Number of Residues
| 405 |
| Molecular Weight
| 41259.91 |
| Theoretical pI
| 6.861 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Cystathionine gamma-lyase
MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEY
SRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRY
FRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHG
DIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGHSDVVMGLVSVNCESLHNRLRFLQ
NSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQH
ELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVL
KNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS
|
| External Links |
|---|
| GenBank ID Protein
| 62898313 |
| UniProtKB/Swiss-Prot ID
| P32929 |
| UniProtKB/Swiss-Prot Entry Name
| CGL_HUMAN |
| PDB IDs
|
|
| GenBank Gene ID
| AK223376 |
| GeneCard ID
| CTH |
| GenAtlas ID
| CTH |
| HGNC ID
| HGNC:2501 |
| References |
|---|
| General References
| Not Available |