| Identification |
|---|
| HMDB Protein ID
| CDBP00341 |
| Secondary Accession Numbers
| Not Available |
| Name
| Carbohydrate sulfotransferase 1 |
| Description
| Not Available |
| Synonyms
|
- GST-1
- Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
- KS6ST
- KSGal6ST
- KSST
- Keratan sulfate Gal-6 sulfotransferase
|
| Gene Name
| CHST1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in sulfotransferase activity |
| Specific Function
| Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.
|
| GO Classification
|
| Biological Process |
| keratan sulfate biosynthetic process |
| galactose metabolic process |
| inflammatory response |
| polysaccharide metabolic process |
| Cellular Component |
| integral to membrane |
| Golgi membrane |
| Component |
| membrane |
| cell part |
| organelle membrane |
| golgi membrane |
| Function |
| catalytic activity |
| transferase activity |
| transferase activity, transferring sulfur-containing groups |
| sulfotransferase activity |
| Molecular Function |
| keratan sulfotransferase activity |
| Process |
| metabolic process |
| primary metabolic process |
| carbohydrate metabolic process |
|
| Cellular Location
|
- Golgi apparatus membrane
- Single-pass type II membrane protein
|
| Pathways
|
| Name | SMPDB/Pathwhiz | KEGG | | Glycosaminoglycan biosynthesis - keratan sulfate | Not Available |  |
|
| Gene Properties |
|---|
| Chromosome Location
| 11 |
| Locus
| 11p11.2 |
| SNPs
| CHST1 |
| Gene Sequence
|
>1236 bp
ATGCAATGTTCCTGGAAGGCCGTCCTCCTCCTTGCCCTGGCCTCCATTGCCATCCAGTAC
ACGGCCATCCGCACCTTCACCGCCAAGTCCTTTCACACCTGCCCCGGGCTGGCAGAGGCC
GGGCTGGCCGAGCGACTGTGCGAGGAGAGCCCCACCTTCGCCTACAACCTCTCCCGCAAG
ACCCACATCCTCATCCTGGCCACCACGCGCAGCGGCTCCTCCTTCGTGGGCCAGCTCTTC
AACCAGCACCTGGACGTCTTCTACCTGTTTGAGCCCCTCTACCACGTCCAGAACACGCTC
ATCCCCCGCTTCACCCAGGGCAAGAGCCCGGCCGACCGGCGGGTCATGCTAGGCGCCAGC
CGCGACCTCCTGCGGAGCCTCTACGACTGCGACCTCTACTTCCTGGAGAACTACATCAAG
CCGCCGCCGGTCAACCACACCACCGACAGGATCTTCCGCCGCGGGGCCAGCCGGGTCCTC
TGCTCCCGGCCTGTGTGCGACCCTCCGGGGCCAGCCGACCTGGTCCTGGAGGAGGGGGAC
TGTGTGCGCAAGTGCGGGCTACTCAACCTGACCGTGGCGGCCGAGGCGTGCCGCGAGCGC
AGCCACGTGGCCATCAAGACGGTGCGCGTGCCCGAGGTGAACGACCTGCGCGCCCTGGTG
GAAGACCCGCGATTAAACCTCAAGGTCATCCAGCTGGTCCGAGACCCCCGCGGCATTCTG
GCTTCGCGCAGCGAGACCTTCCGCGACACGTACCGGCTCTGGCGGCTCTGGTACGGCACC
GGGAGGAAACCCTACAACCTGGACGTGACGCAGCTGACCACGGTGTGCGAGGACTTCTCC
AACTCCGTGTCCACCGGCCTCATGCGGCCCCCGTGGCTCAAGGGCAAGTACATGTTGGTG
CGCTACGAGGACCTGGCTCGGAACCCTATGAAGAAGACCGAGGAGATCTACGGGTTCCTG
GGCATCCCGCTGGACAGCCACGTGGCCCGCTGGATCCAGAACAACACGCGGGGCGACCCC
ACCCTGGGCAAGCACAAATACGGCACCGTGCGAAACTCGGCGGCCACGGCCGAGAAGTGG
CGCTTCCGCCTCTCCTACGACATCGTGGCCTTTGCCCAGAACGCCTGCCAGCAGGTGCTG
GCCCAGCTGGGCTACAAGATCGCCGCCTCGGAGGAGGAGCTGAAGAACCCCTCGGTCAGC
CTGGTGGAGGAGCGGGACTTCCGCCCCTTCTCGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 411 |
| Molecular Weight
| 46714.21 |
| Theoretical pI
| 8.968 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Carbohydrate sulfotransferase 1
MQCSWKAVLLLALASIAIQYTAIRTFTAKSFHTCPGLAEAGLAERLCEESPTFAYNLSRK
THILILATTRSGSSFVGQLFNQHLDVFYLFEPLYHVQNTLIPRFTQGKSPADRRVMLGAS
RDLLRSLYDCDLYFLENYIKPPPVNHTTDRIFRRGASRVLCSRPVCDPPGPADLVLEEGD
CVRKCGLLNLTVAAEACRERSHVAIKTVRVPEVNDLRALVEDPRLNLKVIQLVRDPRGIL
ASRSETFRDTYRLWRLWYGTGRKPYNLDVTQLTTVCEDFSNSVSTGLMRPPWLKGKYMLV
RYEDLARNPMKKTEEIYGFLGIPLDSHVARWIQNNTRGDPTLGKHKYGTVRNSAATAEKW
RFRLSYDIVAFAQNACQQVLAQLGYKIAASEEELKNPSVSLVEERDFRPFS
|
| External Links |
|---|
| GenBank ID Protein
| 2887403 |
| UniProtKB/Swiss-Prot ID
| O43916 |
| UniProtKB/Swiss-Prot Entry Name
| CHST1_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| AB003791 |
| GeneCard ID
| CHST1 |
| GenAtlas ID
| CHST1 |
| HGNC ID
| HGNC:1969 |
| References |
|---|
| General References
| Not Available |